Loading...
|
Please use this identifier to cite or link to this item:
https://ir.csmu.edu.tw:8080/ir/handle/310902500/17842
|
| Title: | The upregulation of transglutaminase-2 by cyclosporin a in human gingival fibroblasts is augmented by oxidative stress |
| Authors: | Lee, Shiuan-Shinn
Tsai, Chung-Hung
Kuan, Yu-Hsiang
Huang, Fu-Mei
Chang, Yu-Chao |
| Contributors: | 中山醫大 |
| Date: | 2012 |
| Issue Date: | 2017-06-28T08:14:25Z (UTC)
|
| Publisher: | Journal of Periodontology |
| ISSN: | 0022-3492 |
| Abstract: | Abstract
BACKGROUND:
Transglutaminase-2 (TGM-2) has been implicated in several fibrotic disorders and can be induced by reactive oxygen species (ROS). Hence, the authors hypothesize that cyclosporin A (CsA) may regulate TGM-2 via ROS, and this regulation may have a role in the pathogenesis of CsA-induced gingival overgrowth.
METHODS:
Cytotoxicity, 2',7'-dichlorodihydrofluorescein diacetate assay, and Western blot were used to investigate the effects of CsA in human gingival fibroblasts (HGFs). In addition, extracellular signal-regulated kinase (ERK) inhibitor PD98059, phosphatidylinositol 3-kinase inhibitor LY294002, glutathione precursor N-acetyl-L-cysteine (NAC), curcumin, epigallocatechin-3 gallate (EGCG), and p38 inhibitor SB203580 were added to find the possible regulatory mechanisms.
RESULTS:
Concentrations of CsA >500 ng/mL demonstrated cytotoxicity to HGFs (P < 0.05). CsA enhanced the generation of intracellular ROS at concentrations >200 ng/mL (P <0.05). TGM-2 protein induced by CsA was found in HGFs in a dose- and time-dependent manner (P <0.05). The addition of PD98059, LY294002, NAC, curcumin, EGCG, and SB203580 markedly inhibited TGM-2 expression induced by CsA (P <0.05).
CONCLUSIONS:
These results demonstrate that CsA significantly upregulates intracellular ROS generation and |
| URI: | http://dx.doi.org/10.1902/jop.2012.120554
https://ir.csmu.edu.tw:8080/ir/handle/310902500/17842 |
| Relation: | Journal of Periodontology October 2013, Vol. 84, No. 10, Pages 1469-1475 |
| Appears in Collections: | [公共衛生學系暨碩士班] 期刊論文
|
Files in This Item:
| File |
Description |
Size | Format | |
|---|
| index.html | | 0Kb | HTML | 242 | View/Open |
|
All items in CSMUIR are protected by copyright, with all rights reserved.
|
