Fascia tissue engineering with human adipose-derived stem cells in a murine model: Implications for pelvic floor reconstruction

doi:10.1016/j.jfma.2013.04.017

中山醫學大學機構典藏 CSMUIR > 醫學院 > 醫學系 > 期刊論文 > Item 310902500/17797

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题名:	Fascia tissue engineering with human adipose-derived stem cells in a murine model: Implications for pelvic floor reconstruction
作者:	Cheng, Yang Vivian Chou, Min-Min Chen, Gin-Den Huang, Ying-Ting Wen, Mei-Chin Hung, Man-Jung
贡献者:	醫學研究所
关键词:	adipose-derived stem cells;fibroblastic differentiation;pelvic floor reconstruction;pelvic organ prolapse;tissue engineering
日期:	2014-10
上传时间:	2017-06-26T08:58:22Z (UTC)
出版者:	Journal of the Formosan Medical Association
ISSN:	0929-6646
摘要:	Background/Purpose Mesh-augmented vaginal surgery for treatment of pelvic organ prolapse (POP) does not meet patients' needs. This study aims to test the hypothesis that fascia tissue engineering using adipose-derived stem cells (ADSCs) might be a potential therapeutic strategy for reconstructing the pelvic floor. Methods Human ADSCs were isolated, differentiated, and characterized in vitro. Both ADSCs and fibroblastic-differentiated ADSCs were used to fabricate tissue-engineered fascia equivalents, which were then transplanted under the back skin of experimental nude mice. Results ADSCs prepared in our laboratory were characterized as a group of mesenchymal stem cells. In vitro fibroblastic differentiation of ADSCs showed significantly increased gene expression of cellular collagen type I and elastin (p < 0.05) concomitantly with morphological changes. By contrast, ADSCs cultured in control medium did not demonstrate these changes. Both of the engrafted fascia equivalents could be traced up to 12 weeks after transplantation in the subsequent animal study. Furthermore, the histological outcomes differed with a thin (111.0 ± 19.8 μm) lamellar connective tissue or a thick (414.3 ± 114.9 μm) adhesive fibrous tissue formation between the transplantation of ADSCs and fibroblastic-differentiated ADSCs, respectively. Nonetheless, the implantation of a scaffold without cell seeding (the control group) resulted in a thin (102.0 ± 17.1 μm) fibrotic band and tissue contracture. Conclusion Our results suggest the ADSC-seeded implant is better than the implant alone in enhancing tissue regeneration after transplantation. ADSCs with or without fibroblastic differentiation might have a potential but different role in fascia tissue engineering to repair POP in the future.
URI:	https://doi.org/10.1016/j.jfma.2013.04.017 https://ir.csmu.edu.tw:8080/ir/handle/310902500/17797
關聯:	Journal of the Formosan Medical Association Volume 113, Issue 10, October 2014, Pages 704-715
显示于类别:	[醫學系] 期刊論文

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