Involvement of mitochondrial pathway in Taxol-induced apoptosis of human T24 bladder cancer cells

doi:10.1007/s00240-002-0263-4

中山醫學大學機構典藏 CSMUIR > 醫學科技學院 > 醫學檢驗暨生物技術學系暨碩士班 > 期刊論文 > Item 310902500/15958

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题名:	Involvement of mitochondrial pathway in Taxol-induced apoptosis of human T24 bladder cancer cells
作者:	SY, Yuan SL, Hsu KJ, Tsai CR, Yang
贡献者:	中山醫學大學
关键词:	Taxol Urothelial cancer Apoptosis Bcl-2 Caspases
日期:	2002
上传时间:	2016-09-06T08:22:59Z (UTC)
ISSN:	0300-5623
摘要:	We examined a human urothelial cancer T24 cell line, which was exposed to clinically achievable concentrations of Taxol and detected the lethal effect of Taxol as measured by a cytotoxic dose-response curve. Marked nuclear condensation and the fragmentation of chromatin were observed by DAPI stain, DNA ladder formation, and flow cytometry at an LC(90)concentration of 0.8 microg/ml Taxol, which also induced a G2/M arrest. In response to Taxol-treatment, caspase-9 activity increased at 8 h, and both caspase-2 and -3 activities were increased twofold relative to control cultures at 16 h. Moreover, treatment with the broad-spectrum caspase inhibitor (z-VAD-fmk) or the caspase-9 specific inhibitor (z-LEHD-fmk) effectively protected T24 cells against Taxol-triggered apoptosis. Furthermore, the phosphorylation of Bcl-2 and Bcl-X(L) proteins in Taxol treated cells was detected at 8 h. In contrast, Taxol had no effect on the levels of Fas and FasL proteins and neither antagonistic, anti-Fas antibody affected Taxol-induced apoptosis. These results suggest that, following the phosphorylation of Bcl-2 and Bcl-X(L)proteins, Taxol-induced apoptosis is induced through the mitochondria-dependent pathway in T24 cells.
URI:	http://dx.doi.org/10.1007/s00240-002-0263-4 https://ir.csmu.edu.tw:8080/ir/handle/310902500/15958
關聯:	Urol Res. 2002 Oct;30(5):282-8. Epub 2002 Sep 19
显示于类别:	[醫學檢驗暨生物技術學系暨碩士班] 期刊論文

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