Development of a portable impedance detection system for monitoring the growth of mouse L929 cells

doi:10.1016/j.jtice.2012.04.008

中山醫學大學機構典藏 CSMUIR > 醫學科技學院 > 醫學檢驗暨生物技術學系暨碩士班 > 期刊論文 > Item 310902500/15946

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/15946

Title:	Development of a portable impedance detection system for monitoring the growth of mouse L929 cells
Authors:	Chen, Ching Jung Liu, Jen Tsai Chang, Shwu-Jen Lee, Ming-Wei Tsai, Jang-Zern
Contributors:	中山醫學大學
Keywords:	Impedance measurement;Interdigitating electrodes;L929 cells;Microfabrication
Date:	2012
Issue Date:	2016-09-06T07:32:15Z (UTC)
ISSN:	1876-1070
Abstract:	A portable impedance measurement system was used to monitor the growth of L929 cells. The interdigitating microelectrodes of our portable system were produced by microfabrication technology with a width of 8 μm, a gap of 8 μm, and a length of 500 μm. These microelectrodes provided a high electric field for AC impedance measurements. When mouse L929 cells were cultured on the electrode surface and pre-coated with poly-l-lysine, the changes in the impedance on the interdigitating electrodes represented the lag, log, and stationary phases of a typical cell growth curve. The impedance measurement result was fitted to an equivalent circuit, which showed that the capacitance signal was the major contributor to the total impedance of the cell culture. The capacitance arises from the cell capacitance, the double-layer capacitance and the capacitance of the poly-l-lysine-coated surface, and the changes in capacitance were probably due to cell proliferation and the resulting changes in cell metabolites. To make the device portable and provide rapid results, we designed a novel impedance detection system comprised of a microprocessor, an AD5933 impedance converter (IC), and the interdigitating microelectrodes. This device was successfully used to observe the changes in the metabolism and proliferation of L929 cells at different stages of cell growth with low cell concentrations. Further, the portable impedance measurement device is a useful analytical tool for monitoring cell growth. It will integrate easily with wireless circuits for applications in aseptic cell biology.
URI:	http://dx.doi.org/10.1016/j.jtice.2012.04.008 https://ir.csmu.edu.tw:8080/ir/handle/310902500/15946
Relation:	Journal of the Taiwan Institute of Chemical Engineers Volume 43, Issue 5, September 2012, Pages 678–684
Appears in Collections:	[醫學檢驗暨生物技術學系暨碩士班] 期刊論文

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