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https://ir.csmu.edu.tw:8080/ir/handle/310902500/15918
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| Title: | Involvement of Viral and Chemical Factors with Oral Cancer in Taiwan |
| Authors: | Yang, Yu-Yen;Koh, Lim-Woh;Tsai, Ju-Hsin;Tsai, Chung-Hung;Wong, Eric Fook-Chuen;Lin, Shyh-Jye;Yang, Chi-Chiang |
| Contributors: | 中山醫學大學 |
| Keywords: | Oral cancer;virus;arecoline |
| Date: | 2004 |
| Issue Date: | 2016-09-05T08:50:05Z (UTC)
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| Abstract: | Background: The association between oral squamous cell carcinoma (OSCC) and viral and
chemical factors is uncertain. Therefore the correlation of viral and chemical factors with oral
cancer in Taiwan was investigated.
Methods: Thirty-seven paraffin-embedded oral cancer biopsies and 36 normal oral tissue
specimens were examined by the polymerase chain reaction method for six viruses: HPV,
CMV, EBV, HSV-1, HSV-2 and HHV-8. To elucidate the role of arecoline in the oncogenesis of
oral cancer, human buccal fibroblasts, oral submucosal fibroblasts and three cancer cell lines
KB, GNM and TSCCa were used for MTT cytotoxity assay and flow cytometry DNA content
analysis.
Results: Two (5.4%) HSV-1-positive and four (10.8%) HPV-positive cases were recognized in
oral cancer biopsies. Among the four HPV-positive tissues, two were further typed as HPV-16,
one was identified as HPV-18- and HSV-1-positive; and one contained both HPV-16 and HPV-
18. One sample presented HSV-1 only. Arecoline, at a concentration lower than 0.8 µg/ml,
increased cell growth (all cell types); at higher concentrations (25–400 µg/ml) it was cytotoxic.
The cell cycle was demonstrated to be altered either by low or high concentrations of arecoline
treatment, depending on the cells treated.
Conclusions: The data demonstrated that HPV, HSV-1 and betel quid chewing were significantly
associated with OSCC, but HSV-2, CMV, EBV and HHV-8 were not. We suggest that the
most determinative factor for oral cancer may be chemical in nature rather than viral infection. |
| URI: | https://ir.csmu.edu.tw:8080/ir/handle/310902500/15918 |
| Relation: | Jpn J Clin Oncol 2004;34(4)176–183 |
| Appears in Collections: | [醫學檢驗暨生物技術學系暨碩士班] 期刊論文
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