This study explored to assess cell viability using a general diabetes blood glucose meter. Cell viabilities were assessed on
the basis of glucose consumption and compared with those measured using a MTT assay. The statistical analysis showed
the consistency of the results of two methods. Cell viability assessments on the basis of the glucose consumption in the
culture medium are suitable for applications of various cancer cell lines and epithelial cell lines. The blood glucometer
meets the needs of instant viability assessment during cell culture with calibration-free, and maintenance-free. This device
detected the cell viability on the basis of the glucose consumption in the culture medium. It is economical and the cultured
cells can be used for further mechanism study of drug effects.