Assessment of the cytotoxicity of chlorhexidine by employing an in vitro mammalian test system

doi:10.1016/j.jds.2013.02.011

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 口腔醫學研究所 > 期刊論文 > Item 310902500/15807

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题名:	Assessment of the cytotoxicity of chlorhexidine by employing an in vitro mammalian test system
作者:	Li, Yi-Ching Kuan, Yu-Hsiang Lee, Tzu-Hsin Huang, Fu-Mei Chang, Yu-Chao
贡献者:	中山醫大口腔科學研究所
关键词:	cell death;chlorhexidine;cytotoxicity;superoxide anion
日期:	2014
上传时间:	2016-08-15T06:13:54Z (UTC)
ISSN:	1991-7902
摘要:	Background/purpose Chlorhexidine (CHX), a chlorophenyl biguanide with broad antibacterial action, has been widely used in dentistry. The initial uses of CHX in dentistry were to wash operation site and to disinfect root canals. Recently, the addition of CHX into many dental materials has improved the overall therapeutic efficacy. The aim of this study was to evaluate the potential toxicological implications of CHX employing an in vitro mammalian test system. Materials and methods Cytotoxicity, mode of cell death, and generation of superoxide anion were performed to elucidate the toxic effects of CHX on Chinese hamster ovary cells. Cytotoxicity was judged using tetrazolium bromide reduction assay. The mode of cell death was determined by flow cytometry. Superoxide anion generation was determined by the superoxide dismutase-inhibitable reduction of ferricytochrome c. Results CHX demonstrated a cytotoxic effect on Chinese hamster ovary cells in a dose-dependent and time-dependent manner (P < 0.05). The mode of cell death changed from apoptosis to necrosis as the concentrations of CHX elevated. CHX demonstrated a significant superoxide anion generation in a dose-dependent manner (P < 0.05). The addition of superoxide dismutase decreased the cytotoxicity induced by CHX (P < 0.05). Conclusion CHX was demonstrated to exhibit cytotoxicity that could disrupt the stable cellular redox balance, resulting in increasing levels of free radical generation and subsequent cell death. CHX has significant potential for cytotoxicity.
URI:	http://dx.doi.org/10.1016/j.jds.2013.02.011 https://ir.csmu.edu.tw:8080/ir/handle/310902500/15807
關聯:	Journal of Dental SciencesVolume 9, Issue 2, June 2014, Pages 130–135
显示于类别:	[口腔醫學研究所] 期刊論文

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