中山醫學大學機構典藏 CSMUIR:Item 310902500/15726
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    請使用永久網址來引用或連結此文件: https://ir.csmu.edu.tw:8080/ir/handle/310902500/15726


    題名: Medulloblastoma-derived tumor stem-like cells acquired resistance to TRAIL-induced apoptosis and radiosensitivity.
    作者: CC, Yu;GY, Chiou;YY, Lee;YL, Chang;PI, Huang;YW, Cheng;LK, Tai;HH, Ku;SH, Chiou;TT, Wong
    貢獻者: 中山醫大口腔科學研究所
    日期: 2010-02-24
    上傳時間: 2016-08-11T03:28:11Z (UTC)
    ISSN: 0256-7040
    摘要: OBJECTS:

    Medulloblastoma (MB) is the most malignant primary brain tumor in early childhood that contains cellular and functional heterogeneity. Recent evidence has demonstrated that the tumor stem cells (TSC) may explain the radiochemoresistance of brain tumors, including MB. The aim of the present study is to investigate the possible role of TNF-related apoptosis-inducing ligand (TRAIL) in viability and tumorigenicity of MB cells and MB-derived TSC.

    METHODS:

    MB-associated TSC were isolated and cultured by serum-free medium with bFGF and EGF. The parental MB cells and MB-TSC cells were treated with TRAIL in different concentrations and assessed for cell viability, invasion ability, colony forming ability, and radiotherapy effect.

    RESULTS:

    We enrich a subpopulation of MB-TSC cells using tumor spheroid formation approach. MB-TSC display enhanced self-renewal and highly expressed "stemness" genes (CD133, Sox-2, Bmi1, Nestin). Additionally, MB-TSC showed significant resistance to TRAIL-induced apoptosis and radiosensitivity compared to the parental MB cells due antiapoptotic gene (c-FLIP, Caspase 8, Bcl-2, and Bax) upregulation.

    CONCLUSIONS:

    Our data suggest that MB-TSC are resistant to TRAIL-induced apoptosis and tumorigenic properties. Understanding the molecular mechanisms by which to operate the physiological characteristics in MB-TSC cells offers attractive approach for MB treatment.
    URI: https://ir.csmu.edu.tw:8080/ir/handle/310902500/15726
    http://dx.doi.org/10.1007/s00381-010-1087-0
    關聯: Childs Nerv Syst. 2010 Jul;26(7):897-904
    顯示於類別:[牙醫學系暨碩士班] 期刊論文

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