中山醫學大學機構典藏 CSMUIR:Item 310902500/15687
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    請使用永久網址來引用或連結此文件: https://ir.csmu.edu.tw:8080/ir/handle/310902500/15687


    題名: Sulforaphane targets cancer stemness and tumor initiating properties in oral squamous cell carcinomas via miR-200c induction.
    作者: CM, Liu;CY, Peng;YW, Liao;MY, Lu;ML, Tsai;JC, Yeh;CH, Yu;CC, Yu
    貢獻者: 中山醫大口腔科學研究所
    關鍵詞: cancer stemness;oral squamous cell carcinomas;sulforaphane
    日期: 2016-02-12
    上傳時間: 2016-08-09T07:44:31Z (UTC)
    ISSN: 0929-6646
    摘要: Cancer stem cells (CSCs) are deemed as the driving force of tumorigenesis in oral squamous cell carcinomas (OSCCs). In this study, we investigated the chemotherapeutic effect of sulforaphane, a dietary component from broccoli sprouts, on targeting OSCC-CSCs.
    METHODS:
    The effect of sulforaphane on normal oral epithelial cells (SG) and sphere-forming OSCC-CSCs isolated from SAS and GNM cells was examined. ALDH1 activity and CD44 positivity of OSCC-CSCs with sulforaphane treatment was assessed by flow cytometry analysis. In vitro and in vivo tumorigenicity assays of OSCC-CSCs with sulforaphane treatment were presented.
    RESULTS:
    We observed that the sulforaphane dose-dependently eliminated the proliferation rate of OSCC-CSCs, whereas the inhibition on SG cells proliferation was limited. Cancer stemness properties including self-renewal, CD44 positivity, and ALDH1 activity were also decreased in OSCC-CSCs with different doses of sulforaphane treatment. Moreover, sulforaphane treatment of OSCC-CSCs decreased the migration, invasion, clonogenicity, and in vivo tumorigenicity of xenograghts. Sulforaphane treatment resulted in a dose-dependent increase in the levels of tumor suppressive miR200c.
    CONCLUSION:
    These lines of evidence suggest that sulforaphane can suppress the cancer stemness and tumor-initiating properties in OSCC-CSCs both in vitro and in vivo.
    URI: https://ir.csmu.edu.tw:8080/ir/handle/310902500/15687
    http://dx.doi.org/10.1016/j.jfma.2016.01.004
    關聯: J Formos Med Assoc. 2016 Feb 12.
    顯示於類別:[牙醫學系暨碩士班] 期刊論文

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