Nelumbo nucifera Gaertn leaves extract inhibits the angiogenesis and metastasis of breast cancer cells by downregulation connective tissue growth factor (CTGF) mediated PI3K/AKT/ERK signaling

doi:10.1016/j.jep.2016.05.012

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 期刊論文 > Item 310902500/15620

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/15620

Title:	Nelumbo nucifera Gaertn leaves extract inhibits the angiogenesis and metastasis of breast cancer cells by downregulation connective tissue growth factor (CTGF) mediated PI3K/AKT/ERK signaling
Authors:	Chang, C.-H. Ou, T.-T. Yang, M.-Y. Huang, C.-C. Wang, C.-J.
Contributors:	醫學研究所
Keywords:	Angiogenesis;Breast cancer;Connective tissue growth factor;Invasiveness;Nelumbo nucifera Gaertn
Date:	2016-07-21
Issue Date:	2016-08-04T04:17:32Z (UTC)
Publisher:	Elsevier Ireland Ltd
ISSN:	03788741
Abstract:	Ethnopharmacological relevance Nelumbo nucifera Gaertn (Nymphaeaceae) has been recognized as a medicinal plant, which was distributed throughout the Asia. The aqueous extract of Nelumbo nucifera leaves extract (NLE) has various biologically active components such as polyphenols, flavonoids, oligomeric procyanidines. However, the role of NLE in breast cancer therapy is poorly understood. The aim of this study The purpose of this study was to identify the hypothesis that NLE can suppress tumor angiogenesis and metastasis through CTGF (connective tissue growth factor), which has been implicated in tumor angiogenesis and progression in breast cancer MDA-MB-231 cells. Results We examined the effects of NLE on angiogenesis in the chicken chorioallantoic membrane (CAM) model. The data showed that NLE could reduce the chorionic plexus at day 17 in CAM and the duration of this inhibition was dose-dependent. In Xenograft model, NLE treatment significantly reduced tumor weight and CD31 (capillary density) over control, respectively. We examined the role of angiogenesis involved restructuring of endothelium using human umbilical vein endothelial cell (HUVEC) in Matrigel angiogenesis model. The results indicated that vascular-like structure formation was further blocked by NLE treatment. Moreover, knockdown of CTGF expression markedly reduced the expression of MMP2 as well as VEGF, and attenuated PI3K-AKT-ERK activation, indication that these signaling pathways are crucial in mediating CTGF function. Conclusion The present results suggest that NLE might be useful for treatment in therapy-resistance triple negative breast cancer. © 2016 Elsevier Ireland Ltd.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/15620 http://dx.doi.org/10.1016/j.jep.2016.05.012
Relation:	Journal of Ethnopharmacology Volume 188, 21 July 2016, Pages 111-122
Appears in Collections:	[生化微生物免疫研究所] 期刊論文

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