Epicatechin-3-gallate reverses TGF-β1-induced epithelial-to-mesenchymal transition and inhibits cell invasion and protease activities in human lung cancer cells

doi:10.1016/j.fct.2016.05.009

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 期刊論文 > Item 310902500/15614

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/15614

Title:	Epicatechin-3-gallate reverses TGF-β1-induced epithelial-to-mesenchymal transition and inhibits cell invasion and protease activities in human lung cancer cells
Authors:	Huang, S.-F. Horng, C.-T. Hsieh, Y.-S. Hsieh, Y.-H. Chu, S.-C. Chen, P.-N.
Contributors:	醫學研究所
Keywords:	ECG;EMT;Lung cancer;Metastasis;Polyphenol
Date:	2016-08-01
Issue Date:	2016-08-04T03:51:54Z (UTC)
Publisher:	Elsevier Ltd
ISSN:	02786915
Abstract:	Epithelial-to-mesenchymal transition (EMT) and invasion potential have been considered as essential factors in cancer metastasis, which is the major cause of cancer death. EMT is a multi-step process that involves gain invasion, cytoskeleton change, cell adhesion, and proteolytic extracellular matrix degradation. Epicatechin-3-gallate (ECG), which is a natural polyphenolic component of green tea, elicits several antioxidant and anti-inflammatory effects. However, the effects of ECG on cancer invasion and EMT of human lung carcinoma remain unknown. We provided molecular evidence supporting the anti-metastatic effect of ECG. This compound suppressed the invasion (P < 0.001) of highly metastatic A549 cells by reducing the activities of matrix metalloproteinase-2 (P < 0.001) and urokinasetype plasminogen activator (P < 0.001). ECG also reversed the transforming growth factor (TGF)-β1-induced EMT and upregulated epithelial markers, such as E-cadherin. Conversely, ECG inhibited mesenchymal markers, such as fibronectin and p-FAK. The subcutaneous inoculation of this compound also inhibited the tumor growth of the A549 cells in vivo. Therefore, ECG may be used as an anti-cancer and anti-invasion agent for the adjuvant treatment and metastasis control of human lung cancer cells. ECG may also be administered as an effective chemopreventive agent against TGF-β1-induced EMT. © 2016 Elsevier Ltd.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/15614 http://dx.doi.org/10.1016/j.fct.2016.05.009
Relation:	Food and Chemical Toxicology Volume 94, 1 August 2016, Pages 1-10
Appears in Collections:	[生化微生物免疫研究所] 期刊論文

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