Delphinidin-rich extracts of Hibiscus sabdariffa L. trigger mitochondria-derived autophagy and necrosis through reactive oxygen species in human breast cancer cells

doi:10.1016/j.jff.2016.05.018

中山醫學大學機構典藏 CSMUIR > 醫學院 > 醫學研究所 > 期刊論文 > Item 310902500/15607

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/15607

Title:	Delphinidin-rich extracts of Hibiscus sabdariffa L. trigger mitochondria-derived autophagy and necrosis through reactive oxygen species in human breast cancer cells
Authors:	Wu, C.-H. Huang, C.-C. Hung, C.-H. Yao, F.-Y. Wang, C.-J. Chang, Y.-C.
Contributors:	醫學研究所
Keywords:	Anthocyanins;Autophagy;Breast cancer;Hibiscus sabdariffa L.;Necrosis;Reactive oxygen species
Date:	2016-08-01
Issue Date:	2016-08-04T03:34:53Z (UTC)
Publisher:	Elsevier Ltd
ISSN:	17564646
Abstract:	Anthocyanins from Hibiscus sabdariffa L. (HAs) possess anticancer potential and diverse biological effects, but its correlation with human breast cancer cells and different modes of cell death is unclear. Here, we show that HAs can induce both autophagy and necrosis cell death distinct from apoptosis in MCF-7 cells. HAs could effectively reduce the viability of MCF-7 cells. Unexpectedly, HAs failed to stimulate the apoptosis-related proteins Bcl2 and Bax. Autophagy was characterized by acridine orange staining, LC3 (light chain 3) activation and AMPK (AMP-activated kinase) phosphorylation. The necrosis death was confirmed by annexin V/propidium iodide (PI) double-positive detection and PI uptake assay. HAs-elicited responses were accompanied by reactive oxygen species (ROS) elevation and mitochondrial membrane potential collapse. Moreover, N-acetylcysteine (NAC) reversed HAs induced cell viability. Our study is the first to report that HAs-modified mitochondrial function triggers cell death by autophagy and necrosis in MCF-7 cells rather than programmed cell death. © 2016 Elsevier Ltd.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/15607 http://dx.doi.org/10.1016/j.jff.2016.05.018
Relation:	Journal of Functional Foods Volume 25, 1 August 2016, Pages 279-290
Appears in Collections:	[醫學研究所] 期刊論文

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