脂多醣（Lipopolysaccharide；LPS）為格蘭氏陰性細菌細胞壁外膜的成份，它可以活化單核球及巨噬細胞。在LPS刺激後可引發巨噬細胞分泌一氧化氮以及一些不同的細胞激素，並使其細胞型態改變進而影響巨噬細胞的移動與吞噬作用。薑黃素是一個在東方國家很常見的食物，可用來當抗發炎藥物。在本論文中，我們發現薑黃素可抑制LPS所調控RAW264.7細胞株與老鼠腹腔的巨噬細胞型態的改變及其移動能力的下降，同時這個現象也會造成LPS所引發整體蛋白的酪胺酸磷酸化降低。有趣的是我們也發現薑黃素可抑制巨噬細胞因LPS刺激而增加的c-Src蛋白量。據我們所知，這是第一篇有關於薑黃素藉由抑制c-Src蛋白量進而降低LPS所誘導的巨噬細胞移行之研究。 Lipopolysaccharide (LPS), an outer membrane component of Gram-negative bacteria, is a potent activator of monocytes and macrophages. Upon LPS stimulation, macrophages are provoked to secrete reactive nitrite, and a variety of cytokines, to undergo morphological alteration that results in migration, and to proceed phagocytosis. As a popular dietary spice in the East, curcumin is also a well-known anti-inflammatory agent. In this study, we observed that curcumin inhibited the LPS-meditated morphological alteration that culminated to cellular migration in both Raw264.7 cells and peritoneal macrophages. Concurrent to this phenomenon was the abrogation of LPS-elicited protein tyrosyl phosphorylation upon curcumin exposure. Intriguingly, curcumin could inhibit the LPS-induced c-Src expression that believed to contribute to macrophage activation. To our knowledge, this is the first report that curcumin can suppress LPS-elicited macrophage migration via attenuation of c-Src induction mediated by LPS.
