口腔癌及其癌前病變第二型環氧合之表現與蜂膠對第二型環氧合抑制之研究

中山醫學大學機構典藏 CSMUIR > 健康管理學院 > 營養學系暨碩士班 > 博碩士論文 > Item 310902500/1341

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题名:	口腔癌及其癌前病變第二型環氧合之表現與蜂膠對第二型環氧合抑制之研究 The COX-2 expression of oral cancer and its precancerous lesions and the inhibition of propolis on COX-2
作者:	紀欣怡 Hsin-I Chi
贡献者:	中山醫學院：營養研究所;王進崑
关键词:	第二型環氧合;口腔粘膜下纖維化;白斑症;口腔癌;蜂膠 COX-2;OSF;leukoplakia;oral cancer;propolis
日期:	2001
上传时间:	2010-05-04T03:03:21Z (UTC)
摘要:	口腔癌為台灣十大癌症之一。本研究之目的在於了解口腔癌及其前期病變之COX-2活性及表現，並評估蜂膠是否具抑制COX-2表現之能力，進而預防口腔癌之發生。以Western blot 觀察口腔粘膜下纖維化 (OSF)、白斑症 (Leukoplakia)及口腔癌的組織中COX-2的表現，結果發現，口腔癌組織中COX-2表現強度最高，其次為白斑，OSF最低。將各種口腔病變細分成輕、中及重等三種程度，則發現COX-2表現強度與口腔病變之嚴重性成正相關。以免疫組織法觀察組織中COX-2表現之情形，發現口腔癌組顯著地高於白斑組及OSF組，另外，口腔癌病變組織較周圍的發炎組織有較顯著之COX-2蛋白質表現。以50ng/ml TPA (12-o-teradecanoylphorbol-13-acetate) 可誘使口腔鱗狀上皮細胞 (KB cell) COX-2之蛋白質過度表現，而在高劑量的蜂膠萃取物處理下，則能明顯抑制COX-2蛋白質的表現。由本研究之結果可知，口腔癌前病變中，可能以白斑癌化之機會最大，其次為OSF。此外，蜂膠具有抑制COX-2之效果，對於口腔癌之預防可能具有功效，但仍有待進一步探討。 Oral cancer has been one of the ten major cancers in Taiwan. This study was to know the COX-2 expression in precancerous lesions tissue and to evaluated the inhibition of propolis on COX-2 expression. By Western blot assay, the COX-2 expression was great in the oral cancer tissue followed by leukoplakia and OSF. The levels of COX-2 expression also showed positive correlation with the severity of each lesions. Immunohistochemistry analysis showed that COX-2 expression in oral cancer tissue was very strong and much higher than in leukoplakia and OSF tissue. Furthermore, Oral cancer tissue indicated higher COX-2 level when compared to its adjacent inflammatory tissue. In vitro study demonstrated that proplis markedly suppressed COX-2 expression of KB cell which was induced by TPA (50ng/ml). All the results showed that moderate leukoplakia could play an important role in the malignant mutation. Moreover, the COX-2 expression of KB cell was decreased by propolis extract. This also suggested that propolis extract was probably a chemopreventive material for oral cancer.
URI:	http://140.128.138.153:8080/handle/310902500/1341
显示于类别:	[營養學系暨碩士班] 博碩士論文

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