本研究主要是探討不同表面粗糙度的鈦（Ti）及鈦六鋁四釩（Ti6Al4V）合金對類造骨細胞（U-2 OS）貼附及增殖的影響。Ti及Ti6Al4V試片經不同號數砂紙研磨（#120、#600及#1500）及1 ?m氧化鋁粉拋光後，利用表面粗度儀（profilometry）測量材料表面粗糙度，利用原子力顯微鏡（atomic force microscope）觀察材料表面立體的形態。細胞成長方面，利用MTT（3-[4,5- dimethylthiazol-2- yl]-2,5- diphenyltetrazolium bromide）分析法評估細胞貼附（二小時）數量，利用台盤藍排除法（trypan blue exclusion method）計算細胞增殖（三天）數目，利用結晶紫染色（crystal violet stain）技術及掃描式電子顯微鏡（scanning electron microscopy）分析及觀察細胞的形態。本研究結果顯示，表面粗糙度會影響U-2 OS細胞在Ti及Ti6Al4V合金表面初期的貼附數目及形態，材料種類（Ti及Ti6Al4V合金）會影響U-2 OS細胞在材料表面的增殖行為。
The effect of surface roughness of Ti and Ti6Al4V alloy on the adhesion and proliferation behavior of osteoblast-like cell (U-2 OS) was investigated. The surface roughnesses (Ra), measured by profilometry, of Ti and Ti6Al4V alloy were in the range of 0.072~0.982 mm and 0.042~0.672 mm, respectively. 3-D surface topography was measured using atomic force microscope, respectively. MTT ( 3- [4,5 - dimethylthiazol - 2- yl ] - 2,5 - diphenyltetrazolium bromide) assay and trypan blue exclusion method were used to calculate the number of cells attached after 2 h and 3 d incubation, respectively. Crystal violet stain technique and scanning electron microscope were used to characterize the cell morphology after 2 h and 3 d incubation. The results of this study showed that surface roughness had a significant effect on the cell morphology and cell adhesion number after 2 h incubation, while the material (Ti and Ti6Al4V) had a significant influence on the cell proliferation number after 3 d incubation.
