中山醫學大學機構典藏 CSMUIR:Item 310902500/12220
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    題名: 荔枝花萃取物改善亞硒酸鈉誘導之白內障形成
    Lychee Flower Extract Prevents Selenite-Induced Cataract Formation
    作者: 林羿陞
    Lin, Yi-Sheng
    貢獻者: 中山醫學大學:生物醫學科學學系碩士班;曾榮凱
    關鍵詞: 白內障;荔枝花;亞硒酸鈉
    cataract;lychee flower;sodium selenite
    日期: 2015
    上傳時間: 2015-09-21T03:02:05Z (UTC)
    摘要: 白內障為目前全球致盲主要原因之一，全球目前共有超過5000萬人罹患白內障，目前部分研究利用天然抗氧化物(antioxidant)來預防或延緩白內障發生，希望藉此提高老年人與糖尿病患者的生活品質。本研究以10 mM的亞硒酸鈉（sodium selenite，Se組）濃度誘導豬水晶體產生白內障，並添加荔枝花萃取液(lychee flower extract, LFE)濃度分別為1 mM (LFE 1組)、5 mM (LFE 5組)、10 mM (LFE 10組)於水晶體培養液中，除觀察記錄水晶體組織變化，更進一步利用質地分析(texture profile analysis, TPA)客觀評估水晶體硬度，同時也檢測總抗氧化力（trolox equivalent antioxidant capacity，TEAC）、超氧化物歧化酶（superoxide dismutase, SOD）、丙二醛（malondialdehyde, MDA）。結果顯示，以顯微鏡觀察發現LFE 10組之水晶體僅皮質部份發生些許霧化，其核心部分仍保持透明，相較經亞硒酸鈉處理未添加任何添加荔枝花萃取液之Se組，明顯發現荔枝花萃取液減緩水晶體於形態之變化，同時TPA之硬度評估亦顯示荔枝花萃取液延緩白內障之硬度變化。而氧化壓力分析結果顯示，LFE 10組之TEAC數值顯著優於Se組(29.35±1.66 uM/mg protein vs. 14.42±3.47 uM/mg protein; p<0.05)，SOD結果表現也是LFE 10組(7.8±0.14 U/mg protein)明顯高於Se組(1.44±0.15 U/mg protein)（P<0.05）；相對的，LFE 10組之MDA表現量則顯著低於Se組(3.39±0.17 um/mg protein vs. 6.8±0.71 um/mg protein; p<0.05)。根據結果得知，於亞硒酸鈉體外誘導水晶體形成白內障之模式可有效利用荔枝花萃取液降低氧化壓力之傷害，進而達到減緩白內障之進程之目的。
    Cataract is the world''s leading causes of blindness. There are more than 50 million people worldwide currently suffer from cataracts. In previous studies show natural antioxidants can prevent or delay the process of cataract formation, thereby improving the quality of life for the elderly and patients with diabetes. In this study, cataract induced by 10 mM of sodium selenite (Se group) in porcine lens were cultured with 1 mM, 5 mM, 10mM lychee flower extract (LFE 1 group; LFE 5 group; LFE 10 group). Morphology of lens in different groups was observed under microscope. Also, the hardness of lens was analyzed by (texture profile analysis, TPA). In addition, the parameters of oxidative stress including trolox equivalent antioxidant capacity (TEAC), superoxide dismutase (SOD), malondialdehyde (MDA) were evaluated. The result of morphology shows that transparency in LFE 10 group is obvious better than Se group. In texture profile analysis, the degree of hardness in Se group is significant higher than that in LFE 10 group (p<0.05). In addition, TEAC and SOD levels in LFE 10 group are significant higher than those in Se group (TEAC: 29.35 ± 1.66 10 vs. 14.42 ± 3.47 uM/mg protein; SOD: 7.8 ± 0.14 vs. 1.44 ± 0.15 U/mg protein; p<0.05). MDA level in LFE 10 group are significant lower than that in Se group (3.39 ± 0.17 vs. 6.8 ± 0.71 um/mg protein; p<0.05). In conclusion, lychee flower extract declines the oxidative stress to prevent or delay the process of cataract formation in in vitro induction model by sodium selenite.
    URI: https://ir.csmu.edu.tw:8080/ir/handle/310902500/12220
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