無毒農法栽種之龍葵水萃取物抗LDL氧化作用 及抗動脈粥狀硬化之研究

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 博碩士論文 > Item 310902500/12196

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Title:	無毒農法栽種之龍葵水萃取物抗LDL氧化作用及抗動脈粥狀硬化之研究 Studies on the effect of water extracts of non-toxic planting Solanum nigrum in anti-LDL oxidation and anti-atherosclerosis
Authors:	李俊毅 Li, Chun-I
Contributors:	中山醫學大學:生化暨生物科技研究所;李彗禎
Keywords:	龍葵;健康食品;動脈粥狀硬化;無毒栽種;氧化型低密度脂蛋白 Solanum nigrum;healthy food;atherosclerosis;non-toxic planting;oxidative low density lipoprotein
Date:	2015
Issue Date:	2015-09-21T02:53:11Z (UTC)
Abstract:	龍葵 (Solanum nigrum L.)為野生草本植物，在台灣全年皆可生長，含有多酚成份並已有多項抗癌及抗氧化之報告，但野生龍葵之產量及品質管控不易，亟待克服。在我們先前的試驗中發現龍葵水萃取物 (SNE)具抗LDL氧化作用及動物中之atherosclerosis，機轉則包含抑制血管平滑肌細胞移行的作用。因此，SNE具發展為一種預防心血管疾病之保健食品的潛能。本年計畫中，我們與焌達科技合作，成功將野生龍葵移至畦間，並以無毒栽種農法種植，計算出葉片採收量為每平方公尺102-115公克，經業界SGS檢測無重金屬及化學農肥料殘留後，萃取其水萃物 (ntSNE) 進行研究。結果發現其多酚成分包含有caffeic acid、rutin、及gallic acid等。將ntSNE進行抗oxidative LDL (oxLDL) 能力的測試，結果發現在試管中能有效減少oxLDL的氧化作用。我們以高脂質飲食誘導紐西蘭大白兔產生動脈粥狀硬化，結果發現ntSNE能延緩動脈粥狀硬化的形成。在抗動脈硬化機轉的探討中，也發現ntSNE能抑制血管平滑肌細胞移行的作用。由以上研究得知ntSNE具有抗oxLDL 及抗動脈粥狀硬化的效用，並可抑制平滑肌細胞移行。我們目前已初步落實先前實驗室對龍葵研究的成果，把龍葵抗動脈粥狀硬化的作用推衍至產業界應用，且以功能性成分作為栽種品質控管的指標，並藉由業界的專業技術，獲得產量穩定且優質的龍葵原料，以產學合作方式將龍葵開發為預防心血管疾病的健康食品。 Solanum nigrumL. is a wildherbaceous plantgrowing entire year in Taiwan. Previous reports showed it contains polyphenols and possesses the ability of anticancer and antioxidation. However, it should be overcome due to the quantity and quality of output being difficult to control. In our previous study, the resultsrevealed that the water extract of Solanum nigrumL. (SNE)have the ability of antioxidation in low density lipoprotein and anti-atherosclerosis; the mechanism might involve the inhibition of blood vessel smooth muscle cell migration. Therefore, SN has the potential to develop a healthy food in preventing cardiovascular disease. The results in this year, we cooperated with the company to move the wild SN to the farm, and plantedit successfully, theproduction rate is about 102-115 grams per mm2. After theSGS detection to make sure no residue of heavy metal and chemical pesticides, the water extract (ntSNE)was extracted for further examination. The major polyphenols existing in ntSNE include caffeic acid, rutin, and gallic acid. In in vitroassay, the results showed ntSNE has the effect in decreasing oxidative LDL (oxLDL). In in vivoassay, high fat diet was used to induce atherosclerosis in Newzealand white rabbits. The results showed that ntSNE possesses the aboility to decelerate the formation of atherosclerosis. In exploring the mechanisms of atherosclerosis, ntSNE can decrease the migration of vascular smooth muscle cell. Taken the above together, ntSNE has the effect to reduce oxLDL and atherosclerosis via deceleratoing the migration of vascular smooth muscle cell. Upon to now, we make the previous results of SN down to earth, and push the results applied in industrial circles. We use its major functional components as index to control the quality, and obtain the stable quantity and good quality of SN based on the professional planting technique in industry. In this study, we develop SN to a healthy food in preventing cardiovascular disease in industry-academic cooperating module.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/12196
Appears in Collections:	[生化微生物免疫研究所] 博碩士論文

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