Influenza-B-virus-induced eye and brain malformations during early chick embryogenesis and localization of the viral RNA in specific areas

doi:10.1007/BF02256570

中山醫學大學機構典藏 CSMUIR > 醫學科技學院 > 醫學檢驗暨生物技術學系暨碩士班 > 期刊論文 > Item 310902500/11881

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题名:	Influenza-B-virus-induced eye and brain malformations during early chick embryogenesis and localization of the viral RNA in specific areas
作者:	Chen, Bo-Yie Chang, Han-Hsin Chiou, Hui-Ling Lin, Dr.David Pei-Cheng
贡献者:	中山醫學大學
日期:	2004
上传时间:	2015-07-30T10:41:05Z (UTC)
ISSN:	1021-7770
摘要:	Influenza is prevalent worldwide, and the teratogenic effects of influenza infection have been suspected to occur within the developing central nervous system. We herein report the sequelae of influenza B viral infection during early chick embryogenesis. Chick embryos at Hamburger-Hamilton stage 9 were infected by an in ovo injection under the blastoderm of influenza B virus (B/Taiwan/25/99). At 48 h after infection, gross malformations of the eye and brain, ranging from 25 to 58% of 168 infected embryos, were observed, in contrast to 3–6% among 71 mock-infected controls (p < 0.0001 for both eye and brain malformations). Histological analyses showed extensive tissue degeneration and aggregates of cells in the head mesenchyme, suggesting cell death and heterotopia. Influenza B viral RNA was directly localized by in situ hybridization with probes specific for the HA segment. Viral RNA was extensively detected in the head surface ectoderm and in the lung bud. In the developing brain, viral RNA was specifically located in the anterior neural retina, habenular area, mid-thalamus, and rhombencephalon. Our data show that influenza B virus can be a teratogenic agent in neural and nonneural embryonic tissues, raising concern for transplacental infection during early pregnancy.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11881 http://dx.doi.org/10.1007/BF02256570
關聯:	Journal of Biomedical Science March–April 2004, Volume 11, Issue 2, pp 266-274
显示于类别:	[醫學檢驗暨生物技術學系暨碩士班] 期刊論文

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