Abrogation of streptococcal pyrogenic exotoxin B-mediated suppression of phagocytosis in U937 cells by Cordyceps sinensis mycelium via production of cytokines.

doi:10.1016/j.fct.2006.08.017

中山醫學大學機構典藏 CSMUIR > 醫學院 > 免疫學研究所 > 期刊論文 > Item 310902500/11778

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11778

Title:	Abrogation of streptococcal pyrogenic exotoxin B-mediated suppression of phagocytosis in U937 cells by Cordyceps sinensis mycelium via production of cytokines.
Authors:	Kuo, CF Chen, CC Lin, CF Jan, MS Huang, RY Luo, YH Chuang, WJ Sheu, CC Lin, YS
Contributors:	中山醫學大學
Keywords:	Cordyceps sinensis mycelium;Streptococcal pyrogenic exotoxin B;Phagocytosis;Cytokines
Date:	2007
Issue Date:	2015-07-29T04:26:20Z (UTC)
ISSN:	0278-6915
Abstract:	Streptococcal pyrogenic exotoxin B (SPE B) is a virulent factor in group A streptococcal infection. We previously showed that SPE B reduced phagocytosis in human monocytic U937 cells. Here we show that the mycelium extract of Cordyceps sinensis (CS), a Chinese immunomodulatory herbal medicine, increased phagocytosis in U937 cells. Neither heat nor trypsin pretreatment prevented CS extract from causing this increase. Further studies indicated that SPE B-mediated suppression of U937 cell phagocytic activity was abrogated by CS extract. Factors in the conditioned medium from CS-extract-treated U937 cells were responsible for blocking the SPE B-mediated suppression of phagocytosis. Heating the conditioned medium eliminated the increase, which suggested that the U937-cell protein products augmented phagocytosis. Analyzing cytokine mRNA expression of U937 cells revealed increases in interferon-gamma (IFN-gamma), interleukin (IL)-12 p35 and p40, and tumor necrosis factor-alpha (TNF-alpha), but not in IL-1beta, IL-6, or IL-8. Treating U937 cells with anti-IFN-gamma, IL-12, and TNF-alpha antibodies also eliminated the conditioned medium-induced increase in phagocytosis. Taken together, SPE B inhibited phagocytosis, but CS mycelium extract abrogated this inhibition by causing cytokine production.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11778 http://dx.doi.org/10.1016/j.fct.2006.08.017
Relation:	Food Chem Toxicol. 2007 Feb;45(2):278-85.
Appears in Collections:	[免疫學研究所] 期刊論文

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