Characterization of membranous and cytoplasmic EGFR expression in human normal renal cortex and renal cell carcinoma

doi:10.1186/1423-0127-16-82

中山醫學大學機構典藏 CSMUIR > 醫學院 > 免疫學研究所 > 期刊論文 > Item 310902500/11710

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题名:	Characterization of membranous and cytoplasmic EGFR expression in human normal renal cortex and renal cell carcinoma
作者:	Pu, Yeong-Shiau Huang, Chao-Yuan Kuo, Yi-Zih Kang, Wang-Yi Liu, Guang-Yaw Huang, A-Mei Yu, Hong-Jeng Lai, Ming-Kuen Huang, Shu-Pin Wu, Wen-Jeng Chiou, Shean-Jaw Hour, Tzyh-Chyuan
贡献者:	中山醫學大學
日期:	2009
上传时间:	2015-07-28T04:44:55Z (UTC)
ISSN:	1021-7770
摘要:	Metastatic renal cell carcinoma (RCC) is highly resistant to conventional systemic treatments, including chemotherapy, radiotherapy and hormonal therapies. Previous studies have shown over-expression of EGFR is associated with high grade tumors and a worse prognosis. Recent studies suggest anticancer therapies targeting the EGFR pathway have shown promising results in clinical trials of RCC patients. Therefore, characterization of the level and localization of EGFR expression in RCC is important for target-dependent therapy. In this study, we investigated the clinical significance of cellular localization of EGFR in human normal renal cortex and RCC. RCC and adjacent normal kidney tissues of 63 patients were obtained for characterization of EGFR expression. EGFR protein expression was assessed by immunohistochemistry on a scale from 0 to 300 (percentage of positive cells × staining intensity) and Western blotting. EGFR membranous staining was significantly stronger in RCC tumors than in normal tissues (P < 0.001). In contrast, EGFR cytoplasmic staining was significantly higher in normal than in tumor tissues (P < 0.001). The levels of membranous or cytoplasmic EGFR expression in RCC tissues were not correlated with sex, tumor grade, TNM stage or overall survival (P > 0.05). These results showed abundant expression of membranous EGFR in RCC, and abundant expression of cytoplasmic EGFR in normal tissues. EGFR expression in RCC was mostly located in the cell membrane, whereas the EGFR expression in normal renal tissues was chiefly seen in cytoplasm. Our results suggest different locations of EGFR expression may be associated with human renal tumorigenesis.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11710 http://dx.doi.org/10.1186/1423-0127-16-82
關聯:	Journal of Biomedical Science 2009, 16:82
显示于类别:	[免疫學研究所] 期刊論文

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