Interleukin-8 can stimulate progesterone secretion from a human trophoblast cell line, BeWo.

doi:10.1290/0404033.1

中山醫學大學機構典藏 CSMUIR > 醫學院 > 醫學系 > 期刊論文 > Item 310902500/11607

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题名:	Interleukin-8 can stimulate progesterone secretion from a human trophoblast cell line, BeWo.
作者:	Tsui, KH Chen, LY Shieh, ML Chang, SP Yuan, CC Li, HY
贡献者:	中山醫學大學
日期:	2004
上传时间:	2015-07-23T10:22:41Z (UTC)
ISSN:	1071-2690
摘要:	Precise paracrine cross-talk between the embryo and the endometrium is essential for the establishment of a successful pregnancy. Previous studies have demonstrated that the expression of interleukin-8 (IL-8) in the endometrium is enhanced during the late-secretory phase and early pregnancy. Furthermore, IL-8 receptor (IL-8R) expression has been detected in trophoblast cells of the developing embryo. To clarify the roles of IL-8 in the endometrium-embryo interactions, the effects of IL-8 on hormone secretion by trophoblast cells were studied using the BeWo trophoblast cell line that retains hormone-secreting properties of normal trophoblast cells. Using reverse transcription-polymerase chain reaction, we found that IL-8R messenger ribonucleic acid (mRNA) was expressed in BeWo cells. The levels of IL-8R mRNA and protein expression in BeWo cells were similar to those in primary first-trimester trophoblast cells. Progesterone (P4) secretion of BeWo cells was comparable with that of first-trimester trophoblast cells but higher than that of third-trimester trophoblast cells. Treatment of BeWo cells with recombinant human IL-8 (rhIL-8) had no effect on cell proliferation, as determined by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Interestingly, secretion of P4, but not human chorionic gonadotropin, from cultured BeWo cells was significantly enhanced when the cells were incubated with rhIL-8. Our results demonstrate that IL-8 may play an important role in the endometrium-embryo interactions by stimulating trophoblast secretion of P4 for maintenance of a successful pregnancy.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11607 http://dx.doi.org/10.1290/0404033.1
關聯:	In Vitro Cell Dev Biol Anim. 2004 Nov-Dec;40(10):331-6.
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