中山醫學大學機構典藏 CSMUIR:Item 310902500/11538
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 17921/22936 (78%)
造訪人次 : 7496625      線上人數 : 551
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋


    請使用永久網址來引用或連結此文件: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11538


    題名: The differential expression of cytosolic carbonic anhydrase in human hepatocellular carcinoma
    作者: Kuo, Wu-Hsien
    Chiang, Whei-Ling
    Yang, Shun-Fa
    Yeh, Kun-Tu
    Yeh, Chung-Min
    Hsieh, Yih-Shou
    Chu, Shu-Chen
    貢獻者: 中山醫學大學
    關鍵詞: Cytosolic carbonic anhydrase;Human hepatocellular carcinomas;Cholangiocellular carcinoma
    日期: 2003
    上傳時間: 2015-07-22T07:49:38Z (UTC)
    ISSN: 0024-3205
    摘要: Cytosolic carbonic anhydrases (CAs), including CAI, CAII and CAIII are present in normal hepatocytes. This study was aimed to investigate the expression status of CAs in hepatocellular carcinomas (HCC) and cholangiocellular carcinoma (CCC) and the role of tumor progression. The activity, protein expression pattern and messenger RNA of cytosolic CA were analyzed by CA activity analysis, immunoblot and RT-PCR in 60 human hepatocellular carcinomas and 10 human cholangiocellular carcinoma surgical specimens. The in situ distribution of CAI, CAII and CAIII in hepatocellular carcinomas tissues were analyzed by immunohistochemistry. The result showed that in each of 60 human hepatocellular carcinomas and 10 cholangiocellular carcinoma, CA activity and protein expression in tumor area was significantly lower than that of paired adjacent normal tissues (P < 0.01), and mRNA expressions in tumor areas were also reduced (P < 0.001). Furthermore, the immunohistochemical studies have further confirmed this reduction of CAI, CAII and CAIII protein expression in tumor areas. There was a statistically significant reduction in the expression of cytosolic CAII in poorly differentiated cancer (P < 0.001). Furthermore, the reduction of CAI, CAII and CAIII in HCC tumor areas was also revealed in this study and this reduction might promote tumor cell motility and contribute to tumor growth and metastasis.
    URI: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11538
    http://dx.doi.org/10.1016/S0024-3205(03)00597-6
    關聯: Life Sciences Volume 73, Issue 17, 12 September 2003, Pages 2211–2223
    顯示於類別:[生化微生物免疫研究所] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    index.html期刊論文0KbHTML404檢視/開啟


    檢視Licence

    SFX Query

    在CSMUIR中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋