PKCalpha expression regulated by Elk-1 and MZF-1 in human HCC cells.

doi:10.1016/j.bbrc.2005.11.015

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 期刊論文 > Item 310902500/11515

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11515

题名:	PKCalpha expression regulated by Elk-1 and MZF-1 in human HCC cells.
作者:	Hsieh, YH Wu, TT Tsai, JH Huang, CY Hsieh, YS Liu, JY
贡献者:	中山醫學大學
关键词:	PKCα;Elk-1;MZF-1;Transcription regulation;Antisense oligonucleotide
日期:	2006
上传时间:	2015-07-22T04:19:16Z (UTC)
ISSN:	0006-291X
摘要:	Our previous study found that PKCalpha was highly expressed in the poor-differentiated human HCC cells and associated with cell migration and invasion. In this study, we further investigated the gene regulation of this enzyme. We showed that PKCalpha expression enhancement in the poor-differentiated human HCC cells was found neither by DNA amplification nor by increasing mRNA stability using differential PCR and mRNA decay assays. After screening seven transcription factors in the putative cis-acting regulatory elements of human PKCalpha promoters, only Elk-1 and MZF-1 antisense oligonucleotide showed a significant reduction in the PKCalpha mRNA level. They also reduced cell proliferation, cell migratory and invasive capabilities, and DNA binding activities in the PKCalpha promoter region. Over-expression assay confirmed that the PKCalpha expression may be modulated by these two factors at the transcriptional level. Therefore, these results may provide a novel mechanism for PKCalpha expression regulation in human HCC cells.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11515 http://dx.doi.org/10.1016/j.bbrc.2005.11.015
關聯:	Biochem Biophys Res Commun. 2006 Jan 6;339(1):217-25. Epub 2005 Nov 10.
显示于类别:	[生化微生物免疫研究所] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html	期刊論文	0Kb	HTML	220	检视/开启

检视Licence

在CSMUIR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....