The in vitro and in vivo apoptotic effects of Mahonia oiwakensis on human lung cancer cells.

doi:10.1016/j.cbi.2009.02.011

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 期刊論文 > Item 310902500/11463

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11463

Title:	The in vitro and in vivo apoptotic effects of Mahonia oiwakensis on human lung cancer cells.
Authors:	Wong, BS Hsiao, YC Lin, TW Chen, KS Chen, PN Kuo, WH Chu, SC Hsieh, YS
Contributors:	中山醫學大學
Keywords:	Mahonia;NSCLC;Chemoprevention;Apoptosis
Date:	2009
Issue Date:	2015-07-21T07:51:53Z (UTC)
ISSN:	0009-2797
Abstract:	Both the root and stem bark of Mahonia species were popular folk medicines. The plant has several proven biological activities including anti-bacterial, anti-fungal, and anti-inflammatory effects. However, Mahonia has not been studied for its anticancer effects. In the present study, we made extracts from Mahonia oiwakensis (MOE), a selected species in Taiwan, and investigated their effects on various human lung cells. We found that MOE-induced apoptotic death in human A549 non-small-cell lung carcinoma (NSCLC) cells in a dose- and time-dependent manner. Treatment with the extracts also caused an increase in the sub-G1 fraction of cells, chromosome condensation, and DNA fragmentation. The mitochondrial-mediated pathway was implicated in this MOE-induced apoptosis as evidenced by the activation of the caspase cascade, cleavage of poly (ADP-ribose) polymerase (PARP), disruption of mitochondrial membrane potential, and release of cytochrome C. A higher ratio of Bax/Bcl-2 proteins and cleavage of Bid were also observed in MOE-induced cell apoptosis. In A549 tumor-xenografted nude mice, MOE also retarded in vivo proliferation (P<0.05) and induced apoptosis in tumor cells, as shown by a decrease in Ki-67-positive staining (P<0.05) and increased transferase-mediated dUTP nick-end labeling (TUNEL)-positive staining (P<0.05). In conclusion, MOE inhibits the growth of human lung cancer cells in vitro and in vivo, suggesting that it may have therapeutic potential against human lung cancer.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11463 http://dx.doi.org/10.1016/j.cbi.2009.02.011
Relation:	Chem Biol Interact. 2009 Jul 15;180(2):165-74.
Appears in Collections:	[生化微生物免疫研究所] 期刊論文

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