| Abstract: | 目的: 研究anti-NS-1及anti-24-peptide抗體在免疫兔子及自體免疫
疾病之角色。
方法: 收集臨床上全身性紅斑狼瘡、類風濕性關節炎、修格連氏症
候群、硬皮症、僵直性脊椎炎及尿毒症等病人179血清及30正
常血清，利用酵素連結免疫分析法分析 B19 抗NS-1及抗24-
peptide IgM及IgG抗體的表現。另取得經NS-1抗原免疫作用
的兔子抗血清，進行proteinase 3(PR3)、myeloperoxidases (MPO)、
E3、cardiolipin、ssDNANS-1等抗原酵素連結免疫分析及吸附試
驗。
結果: 類風濕性關節炎病人抗NS-1 IgM及 IgG抗體陽性比率分別為
31.5%及35.2%，全身性紅斑狼瘡病人則分別為13.1%及15.2%。
類風濕性關節炎病人抗NS-1 IgM及 IgG抗體陽性比率比全身
性紅斑狼瘡病人高。
類風濕性關節炎病人及全身性紅斑狼瘡病人以parvoscreen test
，IgM抗體陽性率各別為27.1%及4.3%，而IgG抗體陽性率則
各別為47.9%及30.4%。parvoscreen test和抗NS-1抗體陽性比
率的結果經卡方檢定(Chi-Square)並無差別。因此，NS-1抗原可
被應用於臨床病人感染 B19的初步篩檢。
兔子經NS-1免疫作用後，抗NS-1抗體除可辨識NS-1抗原外，
尚可辨識如: E3、cardiolipin、ssDNA、MPO及PR3等抗原。抗
NS-1抗體經NS-1抗原吸附作用後，抗NS-1抗體的效價降低，
但辨識E3、PR3自體抗原的能力並未受到抑制。抗NS-1抗體
經E3抗原吸附作用，辨識NS-1及E3、PR3等自體抗原的能力
並未受到抑制。
結論: 類風濕性關節炎病人抗NS-1 IgM及 IgG抗體陽性比全身性紅斑
狼瘡病人高，且NS-1抗原可被應用於臨床病人感染B19的初
步抗體篩檢。
抗NS-1抗體可被 NS-1抗原吸附，但無法被E3抗原所吸附，
由此可推論抗NS-1抗體和E3抗原及PR3抗原有交互作用的現
象。
Objective: To analyze the reactivity of the autoimmune diseases
patients sera with the parvovirus B19 proteins, 24-
peptide and NS-1 was used as solid-phase antigen in
an enzyme linked immunosorbent assay (ELISA) to
screen human sera.
Method : Sera from 80 patients with systemic lupus erythematous
(SLE), 79 patientswith rheumatoid arthritis (RA), 4
patients with Sjogren''s syndrome (SS), 3 patients with
systemic scleroderma (SSc), 4 patients with ankylosing
spinitis (AS), 9 patients with uremia, All the
patients’ sera were tested for B19 infection by using
ELISA. NS-1 and 24-peptide were used to immunize
rabbits. Sera from immunized rabbits were tested for
B19 infection using antigens of NS-1, 24-peptide and
proteinase 3 (PR3) , myeloperoxidases (MPO), E3,
cardiolipin, ssDNA antigens by ELISA. Furthermore,
anti-NS-1 antibodies were preincubated with different
amounts NS-1 or E3 antigens, after antigens-antibodies
absorption, anti-NS-1 antibodies were tested the
direct and comptitive ELISA for antibody activity
against E3, PR3, cardiolipin, ssDNA and MPO antigens.
Results : The IgM and IgG antibodies against NS-1 and VP-1
peptide were higher in patients with RA than SLE .
For example, Anti-NS-1 IgM and IgG antibodies were
31.5% and 35.2% in RA. 13.1% and 15.2% for SLE. For
parvoscreen test which used VP-1 peptides as the
antigens, 27.1% and 47.9% in RA patients. 4.3% and
30.4% for SLE patients. There is no difference
between these two tests as determined by Chi-Square
method. Sera from rabbits immunized with NS-1
antigens, recognize MPO, cardiolipin, E3, PR3, ssDNA
and NS-1 antigens. But the binding of E3 and NS-1
antigens to anti-NS-1 antibodies was not affected
after the antibody was absorpted by E3 antigens.
Conclusion: The prevalence of anti-NS-1 IgM and anti-NS-1 IgG
in patients with RA is higher than those in SLE,
which is probably due to the higher infection rate
of B19 virus in elderly people. Thus, the NS-1
antigens could be used to screen parvovirus B19
infection. Sera from NS-1 immunized animal can
recognize E3, PR3, but these reaction could not be
inhibited by E3 , suggesting that a cross-raction
between the antibodies and the solid-phase antigens
may occur. |
