「培養皿微透析探針」是利用微透析技術直接在細胞培養皿上取樣的專利裝置,改進了以往細胞培養實驗時,取樣過程中繁複、瑣碎的步驟,同時減少在實驗過程中因待測時間的不同,而需同時準備大量細胞培養皿;利用此培養皿微透析探針,可針對同一盤細胞培養皿中之細胞株,細胞培養液中化學物質的變化作一長時間連續偵測,據以了解細胞株生理或病理現象的變異。
應用此一技術,我們探討離體細胞在正常或缺氧的情況下表現的生理或病理反應;從細胞釋放到培養液中的化學物質的含量變化,作為離體細胞從正常趨向病態的指標;本研究結果證實,此項裝置適用於嗜鉻腫瘤細胞及肝臟實質細胞的細胞培養,可同時偵測神經及能量相關化學物質及其代謝物等,以提供細胞培養液中化學成份之動態變化。
本分析技術提供一種快速偵測細胞培養液化學成份的方法, 簡化以往經由放射線酵素標定、化合物萃取、離子交換樹脂或強酸去蛋白過濾等收集樣品再行偵測細胞培養液中化學物質的方式,同時提供「長時間連續性取樣」的優點,以探討離體細胞生理之動態變化。
“Petri dish microdialysis probe” is a pattern device which uses microdialysis technique directly sampling on petri dish. It improves the complicated and trivial procedure during the sampling process while we used to do in cell culture. And, at the time, it reduces the amount of petri dishes according to different testing time. We use the probe to do a long-term continual detecting for the chemical substance changes in the cell culture medium, therefore, to know about the variation of the physiological or pathological phenomena to the cell line.
Applying this technique, we discuss the physiological or pathological reactions in vitro under normal or hypoxic condition and apply this amount changes of the chemical substances, which release from the cell into the medium, as a indicator of the cell changing from normal toward ill status. The experiment results reveals that this device is suitable for the cell culture of pheochromocytoma and primary liver cell, and in the mean while, by detecting the neurotransmitter and energy metabolism. It shows the dynamic changes in the chemical substances of the cell culture medium.
This analysis technique offers us a way to rapidly detect the chemical substance in the cell culture medium. It simplifies the way we used to detecting the chemical substences in the cell medium after sampling via isotop labling, chemical extraction, ion-exchange, or acid deprotin filtration. It advanteges us a long-term, continual sampling method to study the dynamic changes of the physiology of the in-vitro cell.