Detection of Chitinolytic Enzymes in Ipomoea Batatas Leaf Extract by Activity Staining after Gel Electrophoresis

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题名:	Detection of Chitinolytic Enzymes in Ipomoea Batatas Leaf Extract by Activity Staining after Gel Electrophoresis
作者:	Liau, Chun-Yi Lin, Chung-Sheng
贡献者:	中山醫學大學
关键词:	Chitinase;Glycol chitin;Activity staining;Sweet potato
日期:	2008
上传时间:	2015-07-14T10:18:17Z (UTC)
ISSN:	0009-4536
摘要:	A non-denatured SDS-PAGE followed by in-gel activity staining using embedded glycol chitin as a substrate was used to identify the proteins with chitinolyitc activities from sweet potato leaf extract. At least two chitinase activity zones can be clearly identified on the gel at positions with estimated molecular weights of 54.1∼55.6 kDa and 39.6 kDa. Furthermore, our data also indicate that the activity of the larger one can withstand the standard SDS-PAGE sample preparation. Both of these chitinases, however, are different from that of the previously identified chitinase in sweet potato leaves, which has a molecular weight of 16 kDa. By using an embedded substrate, our method has superior sensitivity in detecting chitinases with higher molecular weights. It is a simple, affordable way and may aid in the future discovery of new chitinases.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11254 http://dx.doi.org/10.1002/jccs.200800102
關聯:	Journal of the Chinese Chemical Society Volume 55, Issue 3, pages 678–681, June 2008
显示于类别:	[醫學系] 期刊論文

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