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https://ir.csmu.edu.tw:8080/ir/handle/310902500/11151
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| Title: | Effect of various pulpotomy dressing materials on the viability of L929 cells |
| Authors: | HUANG, TSUI-HSIEN
LIAO, PAO-HSIN
LIU, CHAO-HSIN
YEN, MIN
KAO, CHIA-TZE |
| Contributors: | 中山醫學大學 |
| Keywords: | pulpotomy, biocompatibility, zinc oxide powder, glutaraldehyde, calcium hydroxide formocresol. |
| Date: | 2008 |
| Issue Date: | 2015-07-10T09:35:04Z (UTC)
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| Abstract: | The purpose of the present study was to evaluate the compatibility of mouse periodontal fibroblast
(L929) cells with different formulas of pulpotomy dressing materials. The pulpotomy preparations were
differentiated into group 1: zinc oxide powder, eugenol, and formocresol; group 2: zinc oxide powder,
eugenol, and glutaraldehyde; group 3: zinc oxide powder, eugenol, and ferric sulfate; group 4: calcium
hydroxide, distilled water, and formocresol; group 5: calcium hydroxide, distilled water, and
glutaraldehyde; and group 6: calcium hydroxide, distilled water, and ferric sulfate. All mixed materials
were dissolved in medium and diluted to 10, 20, 40, and 80 µl/ml concentrations. A cell colorimetric assay
(MTT) was used to detect the viability of L929 cells. Results were compared using 1-way analysis of
variance (ANOVA). Differences in treatment means were analyzed using Student-Newman-Keul’s test
and were considered significant at p<0.05. Survival rates of treated L929 cells showed statistical
differences as the concentrations of the pulpotomy materials increased (p < 0.05). The highest survival
rates were found in groups 3, 5, and 6. It is recommended that low-toxicity formulas (such as those
containing zinc oxide powder, eugenol, and ferric sulfate; calcium hydroxide, distilled water, and
glutaraldehyde; or calcium hydroxide, distilled water, and ferric sulfate) be used clinically as pulpotomy
dressing materials. |
| URI: | https://ir.csmu.edu.tw:8080/ir/handle/310902500/11151 |
| Relation: | J Dent Sci, 3(1)：22-29 , 2008 |
| Appears in Collections: | [牙醫學系暨碩士班] 期刊論文
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