The role of integrin αv in proliferation and differentiation of human dental pulp cell response to calcium silicate cement.

doi:10.1016/j.joen.2014.07.016

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/11121

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11121

Title:	The role of integrin αv in proliferation and differentiation of human dental pulp cell response to calcium silicate cement.
Authors:	Hung, CJ HI, Hsu CC, Lin TH, Huang BC, Wu Kao, CT Shie, MY
Contributors:	中山醫學大學
Keywords:	Calcium silicate cement;fibronectin;human dental pulp cell;integrin αv;odontogenesis
Date:	2014
Issue Date:	2015-07-10T05:37:01Z (UTC)
ISSN:	0099-2399
Abstract:	INTRODUCTION: It has been proved that integrin αv activity is related to cell proliferation, differentiation, migration, and organ development. However, the biological functions of integrin αv in human dental pulp cells (hDPCs) cultured on silicate-based materials have not been explored. The aim of this study was to investigate the role of integrin αv in the proliferation and odontogenic differentiation of hDPCs cultured with the effect of calcium silicate (CS) cement and β-tricalcium phosphate (TCP) cement. METHODS: In this study, hDPCs were cultured on CS and TCP materials, and we evaluated fibronectin (FN) secretion and integrin αv expression during the cell attachment stage. After small interfering RNA transfection targeting integrin αv, the proliferation and odontogenesis differentiation behavior of hDPCs were analyzed. RESULTS: The results indicate that CS releases Si ion-increased FN secretion and adsorption, which promote cell attachment more effectively than TCP. The CS cement facilitates FN and αv subintegrin expression. However, the FN adsorption and integrin expression of TCP are similar to that observed in the control dish. Integrin αv small interfering RNA inhibited odontogenic differentiation of hDPCs with the decreased formation of mineralized nodules on CS. It also down-regulated the protein expression of multiple markers of odontogenesis and the expression of dentin sialophosphoprotein protein. CONCLUSIONS: These results establish composition-dependent differences in integrin binding and its effectiveness as a mechanism regulating cellular responses to biomaterial surface. Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11121
Relation:	J Endod. 2014 Nov;40(11):1802-9.
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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