Effect of Verapamil, a Calcium Channel Blocker, on the Odontogenic Activity of Human Dental Pulp Cells Cultured with Silicate-based Materials

doi:10.1016/j.joen.2013.12.019

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/11116

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题名:	Effect of Verapamil, a Calcium Channel Blocker, on the Odontogenic Activity of Human Dental Pulp Cells Cultured with Silicate-based Materials
作者:	Wu, Buor-Chang Kao, Chia-Tze Huang, Tsui-Hsien Hung, Chi-Jr Shie, Ming-You Chung, Hsien-Yang
贡献者:	中山醫學大學
关键词:	Calcium channel blocker;calcium silicate cement;human dental pulp cells;MAPK;odontogenic differentiation;verapamil
日期:	2014
上传时间:	2015-07-10T05:24:07Z (UTC)
ISSN:	0099-2399
摘要:	Introduction This study examines how calcium silicate cement extracts influence the behavior of human dental pulp cells (hDPCs) through calcium channels and active mitogen-activated protein kinase pathways, in particular extracellular signal-related kinase (ERK). Methods HDPCs are treated with various silicon concentrations both with and without verapamil, after which the cells’ viability and odontogenic differentiation markers are determined by using PrestoBlue assay and Western blot, respectively. Results The silicon promoted cell proliferation and inhibited calcium channel blockers. It was also found that silicon increased ERK and p38 activity in a dose-dependent manner. Furthermore, it raised the expression and secretion of alkaline phosphatase, osteocalcin, dentin sialophosphoprotein, and dentin matrix protein-1. In addition, statistically significant differences (P < .05) have been found in the secretion of osteocalcin in ERK inhibitor + verapamil between the silicon concentrations; these varations are dose-dependent and indicate that ERK signaling is involved in the silicon-induced odontogenic differentiation of hDPCs. Conclusions The current study shows that silicon ions released from calcium silicate substrates play a key role in odontoblastic differentiation of hDPCs through calcium channels and modulate ERK activation.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11116 http://dx.doi.org/10.1016/j.joen.2013.12.019
關聯:	Journal of Endodontics Volume 40, Issue 8, August 2014, Pages 1105–1111
显示于类别:	[牙醫學系暨碩士班] 期刊論文

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