A low-level diode laser therapy reduces the lipopolysaccharide (LPS)-induced periodontal ligament cell inflammation

doi:10.1007/s10103-011-1006-y

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/11114

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題名:	A low-level diode laser therapy reduces the lipopolysaccharide (LPS)-induced periodontal ligament cell inflammation
作者:	Huang, T H Chen, C C Liu, S L Lu, Y C Kao, C T
貢獻者:	中山醫學大學
日期:	2014
上傳時間:	2015-07-10T05:15:12Z (UTC)
ISSN:	0268-8921
摘要:	The purpose of this study was to investigate the cytologic effects of inflammatory periodontal ligament cells in vitro after low-level laser therapy. Human periodontal ligament cells were cultured, exposed to lipopolysaccharide and subjected to low-level laser treatment of 5 J cm−2 or 10 J cm−2 using a 920 nm diode laser. A periodontal ligament cell attachment was observed under a microscope, and the cell viability was quantified by a mitochondrial colorimetric assay. Lipopolysaccharide-treated periodontal ligament cells were irradiated with the low-level laser, and the expression levels of several inflammatory markers, iNOS, TNF-α and IL-1, and pErk kinase, were analyzed by reverse transcription polymerase chain reaction and western blot. The data were collected and analyzed by one-way analysis of variance; p < 0.05 indicated a statistically significant difference. The low-level laser treatment of periodontal ligament cells increased their ability to attach and survive. After irradiation, the expression levels of iNOS, TNF-α and IL-1 in lipopolysaccharide-exposed periodontal ligament cells decreased over time (p < 0.05). In periodontal ligament cells, low-level diode laser treatment increased the cells' proliferative ability and decreased the expression of the examined inflammatory mediators.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11114 http://dx.doi.org/10.1007/s10103-011-1006-y
關聯:	T H Huang et al 2014 Laser Phys. Lett. 11 075602
顯示於類別:	[牙醫學系暨碩士班] 期刊論文

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