中山醫學大學機構典藏 CSMUIR:Item 310902500/11114
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    题名: A low-level diode laser therapy reduces the lipopolysaccharide (LPS)-induced periodontal ligament cell inflammation
    作者: Huang, T H
    Chen, C C
    Liu, S L
    Lu, Y C
    Kao, C T
    贡献者: 中山醫學大學
    日期: 2014
    上传时间: 2015-07-10T05:15:12Z (UTC)
    ISSN: 0268-8921
    摘要: The purpose of this study was to investigate the cytologic effects of inflammatory periodontal ligament cells in vitro after low-level laser therapy. Human periodontal ligament cells were cultured, exposed to lipopolysaccharide and subjected to low-level laser treatment of 5 J cm−2 or 10 J cm−2 using a 920 nm diode laser. A periodontal ligament cell attachment was observed under a microscope, and the cell viability was quantified by a mitochondrial colorimetric assay. Lipopolysaccharide-treated periodontal ligament cells were irradiated with the low-level laser, and the expression levels of several inflammatory markers, iNOS, TNF-α and IL-1, and pErk kinase, were analyzed by reverse transcription polymerase chain reaction and western blot. The data were collected and analyzed by one-way analysis of variance; p < 0.05 indicated a statistically significant difference. The low-level laser treatment of periodontal ligament cells increased their ability to attach and survive. After irradiation, the expression levels of iNOS, TNF-α and IL-1 in lipopolysaccharide-exposed periodontal ligament cells decreased over time (p < 0.05). In periodontal ligament cells, low-level diode laser treatment increased the cells' proliferative ability and decreased the expression of the examined inflammatory mediators.
    URI: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11114
    http://dx.doi.org/10.1007/s10103-011-1006-y
    關聯: T H Huang et al 2014 Laser Phys. Lett. 11 075602
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