Evidence for a Na+/Ca2+ exchanger in neuroblastoma x glioma hybrid NG108-15 cells.

doi:10.1042/bj3090445

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 期刊論文 > Item 310902500/11032

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题名:	Evidence for a Na+/Ca2+ exchanger in neuroblastoma x glioma hybrid NG108-15 cells.
作者:	Hsu, LS Chou, WY Chueh, SH
贡献者:	中山醫學大學
日期:	1995
上传时间:	2015-07-03T04:08:25Z (UTC)
ISSN:	0264-6021
摘要:	To determine whether NG108-15 cells contain a functional Na+/Ca2+ exchanger, we isotonically replaced extracellular Na+ with N-methyl-D-glucamine (NMG) and measured the effect on cytosolic Ca2+ concentration ([Ca2+]i) using the fluorescent Ca2+ indicator fura 2. Replacement with NMG alone had no effect on basal [Ca2+]i or the rise in [Ca2+]i evoked by 80 mM K+ or 10 microM bradykinin, but caused a larger [Ca2+]i increase when thapsigargin and carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) were added to the cells; this enhanced [Ca2+]i increase could be reversed by adding Na+ back to the bathing buffer. The elevation in [Ca2+]i induced by thapsigargin and FCCP was inversely proportional to extracellular Na+ concentration. Furthermore, the exchanger operated in the reverse mode, as measured by either [Ca2+]i change or 45Ca2+ uptake. An 810 bp cDNA fragment of the exchanger was amplified by PCR; it differed by a single amino acid residue from the corresponding segment of the rat brain Na+/Ca2+ exchanger. These data suggest that a functioning Na+/Ca2+ exchanger exists in NG108-15 cells.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11032 http://dx.doi.org/10.1042/bj3090445
關聯:	Biochem J. 1995 Jul 15;309 ( Pt 2):445-52.
显示于类别:	[生化微生物免疫研究所] 期刊論文

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