Zebrafish Cyclin-Dependent Protein Kinase–Like 1 (zcdkl1): Identification and Functional Characterization

doi:10.3390/ijms12063606

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 期刊論文 > Item 310902500/11013

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11013

Title:	Zebrafish Cyclin-Dependent Protein Kinase–Like 1 (zcdkl1): Identification and Functional Characterization
Authors:	Hsu, Li-Sung Liang, Cyong-Jhih Tseng, Chen-Yuan Yeh, Chi-Wei Tsai, Jen-Ning
Contributors:	中山醫學大學
Keywords:	cyclin dependent protein kinase-like 1 (cdkl1), expression pattern, floor plate, zebrafish
Date:	2011
Issue Date:	2015-07-02T10:17:37Z (UTC)
ISSN:	1661-6596
Abstract:	The cyclin-dependent protein kinase family regulates a wide range of cellular functions such as cell cycle progression, differentiation, and apoptosis. In this study, we identified a zebrafish cyclin-dependent protein kinase-like 1 protein called zebrafish cdkl1 (zcdkl1), which shared a high degree of homology and conserved synteny with mammalian orthologs. zcdkl1 exhibited abilities for phosphorylation of myelin basic protein and histone H1. RT-PCR analysis revealed that zcdkl1 was expressed starting from fertilization and continuing thereafter. In adult tissues, zcdkl1 was predominantly detected in brain, ovary, and testis, and was expressed at low levels in other tissues. At 50% epiboly stage, zcdkl1 was widely expressed. At 12 to 48 h post-fertilization, zcdkl1 was predominantly expressed in the hypochord, the medial and lateral floor plate, and the pronephric duct. Interference of zcdkl1 expression resulted in abnormalities, such as brain and eye malformation, pericardial edema, and body axis curvature. Disruption of zcdkl1 reduced neurogenin-1 in the brain and sonic hedgehog expression in the floor plate region. These deformities were apparently rescued by co-injection of zcdkl1 mRNA. Findings of this study indicate that zcdkl1 plays an essential role in zebrafish development.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11013 http://dx.doi.org/10.3390/ijms12063606
Relation:	Int J Mol Sci. 2011; 12(6): 3606–3617.
Appears in Collections:	[生化微生物免疫研究所] 期刊論文

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