In vitro and in vivo activity of gallic acid and Toona sinensis leaf extracts against HL-60 human premyelocytic leukemia

doi:10.1016/j.fct.2012.06.046

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 期刊論文 > Item 310902500/11005

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11005

Title:	In vitro and in vivo activity of gallic acid and Toona sinensis leaf extracts against HL-60 human premyelocytic leukemia
Authors:	Huang, Pei-Jane Hseu, You-Cheng Lee, Meng-Shiou Kumar, K.J.Senthil Wu, Chi-R ei Hsu, Li-Sung Liao, Jiunn-Wang Cheng, I-Shiung Kuo, Ya-Ting Huang, Shi-Ying Yang, Hsin-Ling
Contributors:	中山醫學大學
Keywords:	Toona sinensis;Gallic acid;HL-60 cells;Cell-cycle arrest;Xenografted nude mice
Date:	2012
Issue Date:	2015-07-02T09:56:06Z (UTC)
ISSN:	0278-6915
Abstract:	Toona sinensis is one of the most popular vegetarian cuisines in Taiwan and it has been shown to induceapoptosis in cultured human premyelocytic leukemia (HL-60) cells. In the present study, we examinedthe effects of T. sinensis leaf extracts (TS extracts) on tumor regression using in vitro cell culture and an in vivo athymic nude mice model. We found that TS extracts (10–75 l g/mL) arrested HL-60 cells at theG 1 -Stransitionphasethroughthereductions of CyclinD1, CDK4, CyclinE, CDK2, andCyclinA, andinduc-tion of CDK inhibitor p27 KIP levels. Furthermore, VEGF expression and release was signiﬁcantly inhibitedby TS extracts. Notably, TS extracts treatment was effective in terms of delaying tumor incidence in thenude mice inoculated with HL-60 cells as well as reducing the tumor burden. Histological analysis con-ﬁrmed that TS extracts signiﬁcantly modulated tumor progression in xenograft tumor. Furthermore, asimilar pattern of results were observed from gallic acid (5 and 10 l g/mL), a major compound in TS,caused G 1 arrest through regulations of cell-cycle regulatory proteins. Our data suggest that T. sinensis exerts antiproliferative effects onHL-60cells in vitro and in vivo duemainlytothepresence of gallic acid
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11005 http://dx.doi.org/10.1016/j.fct.2012.06.046
Relation:	Food and Chemical Toxicology 50 (2012) 3489–3497
Appears in Collections:	[生化微生物免疫研究所] 期刊論文

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