Rubus idaeus L. reverses epithelial-to-mesenchymal transition and suppresses cell invasion and protease activities by targeting ERK1/2 and FAK pathways in human lung cancer cells.

doi:10.1016/j.fct.2013.10.021

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 期刊論文 > Item 310902500/10998

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题名:	Rubus idaeus L. reverses epithelial-to-mesenchymal transition and suppresses cell invasion and protease activities by targeting ERK1/2 and FAK pathways in human lung cancer cells.
作者:	Hsieh, YS Chu, SC Hsu, LS Chen, KS Lai, MT Yeh, CH Chen, PN
贡献者:	中山醫學大學
关键词:	EMT;FAK;Invasion;MMP;MMP-2;RIE;Rubus idaeus;Rubus idaeus L. extracts;epithelial–mesenchymal transition;focal adhesion kinase;matrix metalloproteinase;u-PA;urokinasetype plasminogen activator
日期:	2013
上传时间:	2015-07-02T09:31:15Z (UTC)
ISSN:	0278-6915
摘要:	Epithelial to mesenchymal transition (EMT) has been considered essential for cancer metastasis, a multistep complicated process including local invasion, intravasation, extravasation, and proliferation at distant sites. Herein we provided molecular evidence associated with the antimetastatic effect of Rubus idaeus L. extracts (RIE) by showing a nearly complete inhibition on the invasion (p<0.001) of highly metastatic A549 cells via reduced activities of matrix metalloproteinase-2 (MMP-2) and urokinasetype plasminogen activator (u-PA). We performed Western blot to find that RIE could induce up-regulation of epithelial marker such as E-cadherin and α-catenin and inhibit the mesenchymal markers such as N-cadherin, fibronectin, snail-1, and vimentin. Selective snail-1 inhibition by snail-1-specific-siRNA also showed increased E-cadherin expression in A549 cells suggesting a possible involvement of snail-1 inhibition in RIE-caused increase in E-cadherin level. RIE also inhibited p-FAK, p-paxillin and AP-1 by Western blot analysis, indicating the anti-EMT effect of RIE in human lung carcinoma. Importantly, an in vivo BALB/c nude mice xenograft model showed that RIE treatment reduced tumor growth by oral gavage, and RIE represent promising candidates for future phytochemical-based mechanistic pathway-targeted cancer prevention strategies.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/10998 http://dx.doi.org/10.1016/j.fct.2013.10.021
關聯:	Food Chem Toxicol. 2013 Dec;62:908-18.
显示于类别:	[生化微生物免疫研究所] 期刊論文

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