Inhibition of protein kinase C and proto-oncogene expression by crocetin in NIH/3T3 cells.

doi:10.1002/(SICI)1098-2744(199612)17:4<235::AID-MC7>3.0.CO;2-C

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 期刊論文 > Item 310902500/10852

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题名:	Inhibition of protein kinase C and proto-oncogene expression by crocetin in NIH/3T3 cells.
作者:	Wang, CJ Cheng, TC Liu, JY Chou, FP Kuo, ML Lin, JK
贡献者:	中山醫學大學
日期:	1996
上传时间:	2015-05-22T07:43:01Z (UTC)
ISSN:	0899-1987
摘要:	Crocetin, a carotenoid isolated from the seeds of Gardenia jasminoides, was found to be a potent inhibitor of tumor promotion induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in mouse skin. When mouse fibroblast NIH/3T3 cells were treated with TPA alone, protein kinase C (PKC) translocated from the cytosolic fraction to the particulate fraction. Pretreatment with 60 and 120 microM crocetin for 15 min inhibited the TPA-induced PKC activity in the particulate fraction by 50% and 66%, respectively, but did not affect the level of PKC protein. Crocetin also reduced the level of TPA-stimulated phosphorylation of cellular proteins. Cells pretreated with crocetin (120 microM) had 55% less PKC [3H]phorbol dibutyrate-binding capacity. Suppression of TPA (100 ng/mL)-induced c-jun and c-fos gene expression was also observed in the mouse fibroblast cells pretreated with crocetin (30, 60, and 120 microM). Our results provided a basis for understanding the inhibitory effect of crocetin on TPA-mediated tumor promotion.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/10852 http://dx.doi.org/10.1002/(SICI)1098-2744(199612)17:4<235::AID-MC7>3.0.CO;2-C
關聯:	Mol Carcinog. 1996 Dec;17(4):235-40.
显示于类别:	[生化微生物免疫研究所] 期刊論文

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