Induction of apoptosis by esculetin in human leukemia cells.

doi:10.1016/S0014-2999(01)00859-7

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 期刊論文 > Item 310902500/10830

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题名:	Induction of apoptosis by esculetin in human leukemia cells.
作者:	Chu, CY Tsai, YY Wang, CJ Lin, WL Tseng, TH
贡献者:	中山醫學大學
关键词:	Apoptosis;Bcl-2;CPP32;Cytochrome c;Esculetin;Leukemia
日期:	2001
上传时间:	2015-05-15T07:38:42Z (UTC)
ISSN:	0014-2999
摘要:	Esculetin, a coumarin compound, has been shown to exhibit antioxidant and anti-inflammatory effects. In the present study, esculetin was found to inhibit the survival of human promyelocytic leukemia HL-60 cells in a concentration-dependent and time-dependent manner. HL-60 cells underwent internucleosomal DNA fragmentation and morphological changes characteristic of apoptosis after a 24-h treatment with esculetin (100 microM). Flow cytometric analysis showed that the hypodiploid nuclei of HL-60 cells were increased to 40.93% after a 36-h treatment with esculetin (100 microM). Further investigation showed that esculetin induced the release of cytochrome c from mitochondria into cytosol in a time-dependent and concentration-dependent manner. Moreover, esculetin application reduced Bcl-2 protein expression to 58% after 9 h as compared with that time at 0. Cysteine protease 32 kDa proenzyme (CPP32), a caspase 3, was activated and its substrate, poly (adenosine diphosphate-ribose) polymerase, was cleaved after a 24-h treatment of HL-60 cells with esculetin. These data suggest that esculetin induces apoptosis in human leukemia cells by increasing cytosolic translocation of cytochrome c and activation of CPP32.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/10830 http://dx.doi.org/10.1016/S0014-2999(01)00859-7
關聯:	Eur J Pharmacol. 2001 Mar 23;416(1-2):25-32.
显示于类别:	[生化微生物免疫研究所] 期刊論文

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