中山醫學大學機構典藏 CSMUIR:Item 310902500/10802
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 17935/22950 (78%)
造访人次 : 7502113      在线人数 : 616
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.csmu.edu.tw:8080/ir/handle/310902500/10802


    题名: Helicobacter pylori promote gastric cancer cells invasion through a NF-kappaB and COX-2-mediated pathway.
    作者: Wu, CY
    Wang, CJ
    Tseng, CC
    Chen, HP
    Wu, MS
    Lin, JT
    Inoue, H
    Chen, GH
    贡献者: 中山醫學大學
    日期: 2005
    上传时间: 2015-05-11T10:34:07Z (UTC)
    摘要: To examine the effects of Helicobacter pylori (H pylori) infection on the invasiveness of gastric cancer cells, and to elucidate its mechanism.
    METHODS:
    Gastric carcinoma cells, MKN-45, were incubated with CagA-positive H pylori, and cell invasion was determined by Matrigel analysis. The expression of matrix metalloproteinase-9 (MMP-9), vascular endothelial growth factor (VEGF), and cyclooxygenase-2 (COX-2) were assessed by Western-blot analysis, and transcriptional activation of the COX-2 promoter was examined by measuring luciferase and beta-galactosidase activities. Lastly, the protein-DNA interaction was confirmed by an electrophoretic mobility shift assay.
    RESULTS:
    The current studies showed that: (1) incubation of CagA-positive H pylori with MKN-45 cells significantly promotes gastric cancer cells invasion, and this effect is attenuated by pre-treatment with NS-398, a COX-2 inhibitor, or PDTC, a nuclear factor kappaB (NF-kappaB) inhibitor; (2) the induction of MKN-45 cells invasion by H pylori is associated with increases in COX-2, MMP-9, and VEGF protein expression, and co-incubation of NS-398 or PDTC significantly reduces these effects; (3) H pylori infection transactivates COX-2 promoter activity and increases the binding of NF-kappaB to this promoter.
    CONCLUSION:
    Our data demonstrate that H pylori infection promotes gastric epithelial cells invasion by activating MMP-9 and VEGF expression. These effects appear to be mediated through a NF-kappaB and COX-2 mediated pathway, as COX-2 or NF-kappaB inhibitor significantly attenuate the invasiveness of gastric cancer cells and the expressions of MMP-9 and VEGF protein.
    URI: https://ir.csmu.edu.tw:8080/ir/handle/310902500/10802
    關聯: World J Gastroenterol. 2005 Jun 7;11(21):3197-203.
    显示于类别:[生化微生物免疫研究所] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    index.html期刊論文0KbHTML269检视/开启


    检视Licence

    SFX Query

    在CSMUIR中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈