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https://ir.csmu.edu.tw:8080/ir/handle/310902500/10802
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| Title: | Helicobacter pylori promote gastric cancer cells invasion through a NF-kappaB and COX-2-mediated pathway. |
| Authors: | Wu, CY
Wang, CJ
Tseng, CC
Chen, HP
Wu, MS
Lin, JT
Inoue, H
Chen, GH |
| Contributors: | 中山醫學大學 |
| Date: | 2005 |
| Issue Date: | 2015-05-11T10:34:07Z (UTC)
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| Abstract: | To examine the effects of Helicobacter pylori (H pylori) infection on the invasiveness of gastric cancer cells, and to elucidate its mechanism.
METHODS:
Gastric carcinoma cells, MKN-45, were incubated with CagA-positive H pylori, and cell invasion was determined by Matrigel analysis. The expression of matrix metalloproteinase-9 (MMP-9), vascular endothelial growth factor (VEGF), and cyclooxygenase-2 (COX-2) were assessed by Western-blot analysis, and transcriptional activation of the COX-2 promoter was examined by measuring luciferase and beta-galactosidase activities. Lastly, the protein-DNA interaction was confirmed by an electrophoretic mobility shift assay.
RESULTS:
The current studies showed that: (1) incubation of CagA-positive H pylori with MKN-45 cells significantly promotes gastric cancer cells invasion, and this effect is attenuated by pre-treatment with NS-398, a COX-2 inhibitor, or PDTC, a nuclear factor kappaB (NF-kappaB) inhibitor; (2) the induction of MKN-45 cells invasion by H pylori is associated with increases in COX-2, MMP-9, and VEGF protein expression, and co-incubation of NS-398 or PDTC significantly reduces these effects; (3) H pylori infection transactivates COX-2 promoter activity and increases the binding of NF-kappaB to this promoter.
CONCLUSION:
Our data demonstrate that H pylori infection promotes gastric epithelial cells invasion by activating MMP-9 and VEGF expression. These effects appear to be mediated through a NF-kappaB and COX-2 mediated pathway, as COX-2 or NF-kappaB inhibitor significantly attenuate the invasiveness of gastric cancer cells and the expressions of MMP-9 and VEGF protein. |
| URI: | https://ir.csmu.edu.tw:8080/ir/handle/310902500/10802 |
| Relation: | World J Gastroenterol. 2005 Jun 7;11(21):3197-203. |
| Appears in Collections: | [生化微生物免疫研究所] 期刊論文
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