|
English
|
正體中文
|
简体中文
|
Items with full text/Total items : 17933/22952 (78%)
Visitors : 7328415
Online Users : 208
|
|
|
Loading...
|
Please use this identifier to cite or link to this item:
https://ir.csmu.edu.tw:8080/ir/handle/310902500/10700
|
Title: | Luteolin enhances paclitaxel-induced apoptosis in human breast cancer MDA-MB-231 cells by blocking STAT3 |
Authors: | Yang, Mon-Yuan Wang, Chau-Jong Chen, Nai-Fang Hoc, Wen-Hsin Lub, Fung-Jou Tseng, Tsui-Hwa |
Contributors: | 中山醫學大學 |
Keywords: | Apoptosis;Fas;Luteolin;Paclitaxel;STAT3 |
Date: | 2013 |
Issue Date: | 2015-05-01T04:18:18Z (UTC)
|
ISSN: | 0009-2797 |
Abstract: | The potential use of low-dose chemotherapy has been appealing because lower dosages are more attainable during cancer therapy and cause less toxicity in patients. Combination therapy of paclitaxel, a promising frontline chemotherapy agent, with natural anti-tumor agents that are considerably less toxic and possess the capability of activating additional apoptotic signals may provide a rational molecular basis for novel chemotherapeutic strategies. Luteolin, a natural flavone, possesses multiple biological activities, including anti-tumor potential. In the present study, the effects of concomitant administration of luteolin and paclitaxel were investigated in human breast cancer MDA-MB-231 cells. Luteolin alone demonstrated an anti-proliferative effect. Co-administration of luteolin and paclitaxel resulted in an increase in apoptosis compared with the treatment of paclitaxel alone as evidenced by the results of a diamidino-2-phenylindole (DAPI) stain and Annexin-V-based assay. Moreover, immunoblotting analysis also showed that the co-administration of luteolin and paclitaxel activated caspase-8 and caspase-3 and increased the expression of Fas. Furthermore, the increased expression of Fas due to co-administration was shown to be due to the blocking of signal transducer and activator of transcription 3 (STAT3). Finally, combination therapy with luteolin and paclitaxel significantly reduced tumor size and tumor weight in an orthotopic tumor model of MDA-MB-231 cells in nude mice. These results suggest that the luteolin–paclitaxel combination could be a novel strategy for the treatment of breast cancer.
Abbreviations
DAPI, diamidino-2-phenylindole; MAPK, mitogen activated protein kinase; PARP, poly (ADP-ribose) polymerase; STAT3, signal transducer and activator of transcription 3 |
URI: | https://ir.csmu.edu.tw:8080/ir/handle/310902500/10700 http://dx.doi.org/10.1016/j.cbi.2014.02.002 |
Relation: | Chemico-Biological Interactions Volume 213, 25 April 2014, Pages 60–68 |
Appears in Collections: | [生化微生物免疫研究所] 期刊論文
|
Files in This Item:
File |
Description |
Size | Format | |
index.html | 期刊論文 | 0Kb | HTML | 381 | View/Open |
|
All items in CSMUIR are protected by copyright, with all rights reserved.
|