Synergistic effects of nicotine on arecoline-induced cytotoxicity in human buccal mucosal fibroblasts.

doi:10.1034/j.1600-0714.2001.030008458.x

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/10636

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/10636

Title:	Synergistic effects of nicotine on arecoline-induced cytotoxicity in human buccal mucosal fibroblasts.
Authors:	Chang, YC Hu, CC Tseng, TH Tai, KW Lii, CK Chou, MY
Contributors:	中山醫學大學
Date:	2001
Issue Date:	2015-04-13T08:58:05Z (UTC)
ISSN:	0904-2512
Abstract:	Areca quid chewing has been linked to oral submucous fibrosis and oral cancer. Arecoline, a major areca nut alkaloid, is considered to be the most important etiologic factor in the areca nut. In order to elucidate the pathobiological effects of arecoline, cytotoxicity assays, cellular glutathione S-transferase (GST) activity and lipid peroxidation assay were employed to investigate cultured human buccal mucosal fibroblasts. To date, there is a large proportion of areca quid chewers who are also smokers. Furthermore, nicotine, the major product of cigarette smoking, was added to test how it modulated the cytotoxicity of arecoline. At a concentration higher than 50 microg/ml, arecoline was shown to be cytotoxic to human buccal fibroblasts in a dose-dependent manner by the alamar blue dye colorimetric assay (P<0.05). In addition, arecoline significantly decreased GST activity in a dose-dependent manner (P<0.05). At concentrations of 100 microg/ml and 400 microg/ml, arecoline reduced GST activity about 21% and 46%, respectively, during a 24 h incubation period. However, arecoline at any test dose did not increase lipid peroxidation in the present human buccal fibroblast test system. The addition of extracellular nicotine acted synergistically on the arecoline-induced cytotoxicity. Arecoline at a concentration of 50 microg/ml caused about 30% of cell death over the 24 h incubation period. However, 2.5 mM nicotine enhanced the cytotoxic response and caused about 50% of cell death on 50 microg/ml arecoline-induced cytotoxicity. Taken together, arecoline may render human buccal mucosal fibroblasts more vulnerable to other reactive agents in cigarettes via GST reduction. The compounds of tobacco products may act synergistically in the pathogenesis of oral mucosal lesions in areca quid chewers. The data presented here may partly explain why patients who combined the habits of areca quid chewing and cigarette smoking are at greater risk of contracting oral cancer.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/10636 http://dx.doi.org/10.1034/j.1600-0714.2001.030008458.x
Relation:	J Oral Pathol Med. 2001 Sep;30(8):458-64.
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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