Paclitaxel induces apoptosis via caspase-3 activation in human osteogenic sarcoma cells (U-2 OS).

doi:10.1016/j.orthres.2005.01.018

中山醫學大學機構典藏 CSMUIR > 醫學院 > 醫學系 > 期刊論文 > Item 310902500/10539

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题名:	Paclitaxel induces apoptosis via caspase-3 activation in human osteogenic sarcoma cells (U-2 OS).
作者:	Lu KH Lue KH Chou MC Chung JG
贡献者:	中山醫學大學
关键词:	Paclitaxel;Osteogenic sarcoma U-2 OS cell;Cell cycle;Apoptosis;Caspase-3
日期:	2005-09
上传时间:	2015-03-23T04:43:10Z (UTC)
ISSN:	0736-0266
摘要:	Paclitaxel has been found to exhibit cytotoxic and antitumor activity. There is little information regarding the mechanisms of apoptotic-inducing effect of paclitaxel on human osteogenic sarcoma U-2 OS cells. Several key regulatory proteins are involved in the initiation of apoptosis. Caspase-3 plays a direct role in proteolytic cleavage of cellular proteins responsible for progression to apoptosis. We examined the effect of paclitaxel on the cell cycle arrest and apoptosis in U-2 OS cells using flow cytometric analysis and Western blotting. We also measured the inhibition of paclitaxel-induced apoptosis and the caspase-3 activity by the broad-spectrum caspase inhibitor z-VAD-fmk on U-2 OS cells. The increased levels of casapse-3 were also confirmed by cDNA microarray. Our observations were: (1) paclitaxel treatment resulted in G2/M-cycle arrest in U-2 OS cells; (2) time and dose dependent apoptosis of U-2 OS cells was induced by paclitaxel; (3) in U-2 OS cells, z-VAD-fmk blocked the paclitaxel-induced apoptosis and caspase-3 activation. These results suggest that paclitaxel-induced G2/M-cycle arrest of the G2/M phase and apoptosis via a caspase-3 pathway in U-2 OS cells.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/10539 http://dx.doi.org/10.1016/j.orthres.2005.01.018
關聯:	J Orthop Res. 2005 Sep;23(5):988-94.
显示于类别:	[醫學系] 期刊論文

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