| Abstract: | 丙酮醛為食品梅納反應中最具活性及毒性之物質,而梅納反應為食品製造過程中常見之非酵素性褐變,懷孕婦女及一般人都很容易暴露在此梅納反應的食品環境中,而導致吸收過多之丙酮醛.丙酮醛對原核細胞及真核細胞
體外培養的毒性已在許多文獻中獲得證實,但其對胚胎的毒性則尚未有相關的報導,故本篇以性腺激素誘發ICR(鼷鼠)小鼠超級排卵,使小鼠受孕後,以二細胞期之鼠胚為材料,再以含有不同濃度之丙酮醛培養基培養鼠胚至孵化,逐日記錄其對胚胎生長發育之影響.根據本實驗結果我們發現,丙酮醛在10^-2mM時可導致鼠胚生長速度與控制組比較有顯著之差異(P<0.05),且丙酮醛對胚胎生長的影響呈現劑量的依存性,丙酮醛於高濃度(1mM)在體
外培養四小時即造成胚胎中細胞形態之改變,此時細胞已無明顯之界限,且呈現顆粒狀物質,繼續觀察則出現兩細胞期阻斷之現象.故丙酮醛若累積至具毒性之劑量時會對胚胎造成形態損傷及生長速度之異常.維生素C是一種
抗氧化劑,且為自由基之清除者;低劑量下除可抗自由基所產生之畸型效應外,又可預防脂質過度氧化所造成之胚胎毒性;但高劑量下對胚胎仍會造成毒性;而本研究發現維生素C之濃度在10^-1mM以上可導致鼠胚生長速度與
控制組比較有顯著之差異(P<0.05);而維生素C(10^-4Mm)在體外培養的第一天可導致鼠胚生長速度增加,低劑量維生素C對胚胎有正面的影響,高劑量維生素C(10^-1mM以上)對胚胎具有毒性,會導致胚胎萎縮及生長停滯,中
低劑量維生素C對胚胎的生長速度及形態皆有正面加強之趨勢,值得更深入探討其對胚胎保護之效果.
ABSTRACTPyruvaldehyde,had been found an important intermediate product of Maillardbrowning reaction.It is a highly active and mutagenetic compound,Pregnantwomen and other people easy to expose to that,susceptible a lot of pyruval-dehyde.However studies of the mutagenetic and genotoxicity of pyruvaldehydehas always been focused on Prokaryotic and Eukaryotic cells.To investigate the suppression of pyruvaldehyde on the development of mouseembryos on preimplantation stage.Two-cell embryos were treated with mediacontaining pyruvaldehyde of different
concentration and observed for 5 days.Growth rate of embryos was depressed in media containing pyruvaldehyde.Microscopic observation indicated that pyruvadehyde induced morphologicalaberation and blocked the development of mouse embryos at concentration above10^-2mM.Vitamin C acts either as a free radical scavenger or as antioxidant.At lowdoes ,Vitamin C can inhibit lipid peroxidation and embryotoxicity.Howere,mightbe
embryotoxicity at higher does of Vitamin C.To investigate the suppression of Vitamin C on the development of mouse embryoson preimplantation stage.Two-cell embryos were treated with media containingVitamin C of different concentration and observed for 5 days.The results show that growth rate of mouse embryos was depressed at Vitamin Chigher than 10^-1 mM.However the development rate of mouse embryos acceleratedwhen the concentration of Vitamin C was lower 10^-4mM.Key words: Pyruvaldehyde ,Vitamin C ,2-cell embryos ,Embryotoxicity. |
