| 摘要: | Salidroside是高山症最常使用的紅景天Rhodiola的萃取純化物，Salidroside是否能抗缺氧 誘發的大腦細胞凋亡仍未知。細胞凋亡途徑主要可分為 Fas death receptor-及 mitochondria- dependent 兩 種 主 要 caspase-dependent 途 徑 外 還 有 Endo G, AIF caspase-independent凋亡途徑，然而是否Salidroside或合併運動治療對睡眠呼吸終止症(夜 間漸歇性缺氧)中大腦細胞Fas& Mitochondrial 凋亡途徑的療效，至今仍未知。 第一年計劃: 為了探討Salidroside和Rhodiola對睡眠呼吸終止症(夜間漸歇性缺氧)中 大腦細胞Fas& Mitochondrial 凋亡和Endo G、AIF凋亡途徑途徑的影響。實驗共分成6組 [G1: Normoxia Control (n=30); G2: Intermittent hypoxia (Hypoxia: 7% O2 60 seconds, 20% O2 alternating 60 seconds, 8 hrs per day, 8 weeks, n=30); G3: Intermittent hypoxia+Salidroside10 (Hypoxia for 8 weeks, hypoxia with pre-treated 10mg/kg salidroside by oral gavage per day for 2nd 4 weeks, n=30); G4: Intermittent hypoxia+Salidroside 30 (Hypoxia for 8 weeks, hypoxia with pre-treated 30mg/kg salidroside by oral gavage per day for 2nd 4 weeks, n=30); G5: Intermittent hypoxia+Rhodiola 90 (Hypoxia for 8 weeks, hypoxia with pre-treated 90 mg/kg Rhodiola crenulata by oral gavage per day for 2nd 4 weeks, n=30); G6: Intermittent hypoxia+Rhodiola 270 (Hypoxia for 8 weeks, hypoxia with pre-treated 270 mg/kg Rhodiola crenulata by oral gavage per day for 2nd 4 weeks, n=30) ] 進 行血壓、多重凋亡相關實驗法例如組織切片、TUNEL assay、western blotting、RT-PCR 等 實驗方法來加以分析並加以探討其大腦細胞凋亡的現象，Fas death receptor-dependent 大 腦 細 胞 凋 亡 (Fas ligand, Fas Death-receptor, caspase 8, and caspase 3) 和 mitochondria-dependent大腦細胞凋亡(Bcl2 family proteins-Bcl2, BNIP3, Bad, cytochrome c release, caspase 9 and caspase 3)和Endo G、AIF等訊息傳遞路徑。 第二年計劃:為了探討運動治療對睡眠呼吸終止症(夜間漸歇性缺氧)對於大腦細胞抗 凋亡和Endo G、AIF凋亡途徑的療效，因此本實驗第二年計劃將分三組 [G1: normoxia Control (n=30); G2: Intermittent hypoxia (Hypoxia: 7% O2 60 seconds, 20% O2 alternating 60 seconds, 8 hrs per day, 8 weeks, n=30); G3: Swimming training+ Intermittent hypoxia (Conditioning 8 weeks with Hypoxia: 7% O2 60 seconds, 20% O2 alternating 60 seconds, 8 hrs per day, 8 weeks, n=30)] 進行血壓、多重凋亡相關實驗法例如大腦組織切片、western blotting、TUNEL assay, RT-PCR 等實驗方法來加以分析並加以探討其大腦細胞凋亡的 現象，Fas death receptor-dependent 大腦細胞凋亡和mitochondria-dependent 大腦細胞凋 亡和Endo G、AIF 等凋亡訊息傳遞路徑。 第三年計劃:為了探討Salidroside配合運動治療是否加乘對睡眠呼吸終止症(夜間漸歇 性缺氧)對於大腦細胞抗凋亡途徑之療效，因此本實驗第三年計劃將分五組[G1: normoxia Control (n=30); G2:Intermittent hypoxia (Hypoxia: 7% O2 60 seconds, 20% O2 alternating 60 seconds, 8 hrs per day, 8 weeks, n=30); G3: Intermittent hypoxia+Salidroside (Hypoxia for 1st 4 weeks, hypoxia pretreated 10mg/kg salidroside by oral gavage per day for 2nd 4 weeks); G4: Swimming training+ Intermittent hypoxia (Conditioning 8 weeks with Hypoxia: 7% O2 60 seconds, 20% O2 alternating 60 seconds, 8 hrs per day, 8 weeks, n=30); G5: Swimming training+ Intermittent hypoxia+ Salidroside (Conditioning 8 weeks+ Hypoxia: 8 weeks and treated 10mg/kg salidroside by oral gavage in 2 nd 4 weeks, 8 weeks, n=30)] 進行血壓、多重 凋亡相關實驗法例如大腦組織切片、western blotting、TUNEL assay, RT-PCR 等實驗方 法來加以分析並加以探討其大腦細胞凋亡的現象。
Salidroside is one of major pure componts of Rhodiola, a traditional herb for high mountain sickness. It is still unknown whether salidroside could prevent intermittent hypoxia induced cortex apoptosis. Apoptosis have two major apoptotic pathways, Fas death receptor-dependent and mitochondria-dependent apoptotic pathways. However, Effects of salidroside and exercise therapy on sleep apnea-induced Cortex apotosis is still totally unknown. The first year: To further clarify the effects of salidroside on cortex apoptosis in sleep apnea. There are six group [G1: Normoxia Control (n=30); G2: Intermittent hypoxia (Hypoxia: 7% O2 60 seconds, 20% O2 alternating 60 seconds, 8 hrs per day, 8 weeks, n=30); G3: Intermittent hypoxia+Salidroside10 (Hypoxia for 8 weeks, hypoxia with pre-treated 10mg/kg salidroside by oral gavage per day for 2nd 4 weeks, n=30); G4: Intermittent hypoxia+Salidroside 30 (Hypoxia for 8 weeks, hypoxia with pre-treated 30mg/kg salidroside by oral gavage per day for 2nd 4 weeks, n=30); G5: Intermittent hypoxia+Rhodiola 90 (Hypoxia for 8 weeks, hypoxia with pre-treated 90 mg/kg Rhodiola crenulata by oral gavage per day for 2nd 4 weeks, n=30); G6: Intermittent hypoxia+Rhodiola 270 (Hypoxia for 8 weeks, hypoxia with pre-treated 270 mg/kg Rhodiola crenulata by oral gavage per day for 2nd 4 weeks, n=30) ]. Blood pressure, Type I cortex apoptosis (Fas ligand, Fas Death-receptor, caspase 8, and caspase 3) and Type II cortex apoptosis (Bcl2 family proteins-Bcl2, BNIP3, Bad, cytochrome c release,caspase 9, and caspase 3), and Endo G/ AIF survival signaling pathways in there four groups will be measured. The second year: To determine the effects of exercise therapy on cortex apoptosis in Sleep apnea. There are three group [G1: normoxia Control (n=30); G2: Intermittent hypoxia (Hypoxia: 7% O2 60 seconds, 20% O2 alternating 60 seconds, 8 hrs per day, 8 weeks, n=30); G3: Swimming training+ Intermittent hypoxia (Conditioning 8 weeks with Hypoxia: 7% O2 60 seconds, 20% O2 alternating 60 seconds, 8 hrs per day, 8 weeks, n=30)]. Blood pressure, cortex Fas and mitochondria dependent apoptotic and Endo G/ AIF survival signaling pathways will be measured. The third year: To determine the effects of exercise training on cortex apoptosis in type II DM. There are five group [G1: normoxia Control (n=30); G2:Intermittent hypoxia (Hypoxia: 7% O2 60 seconds, 20% O2 alternating 60 seconds, 8 hrs per day, 8 weeks, n=30); G3: Intermittent hypoxia+Salidroside (Hypoxia for 1st 4 weeks, hypoxia pretreated 10mg/kg salidroside by oral gavage per day for 2nd 4 weeks); G4: Swimming training+ Intermittent hypoxia (Conditioning 8 weeks with Hypoxia: 7% O2 60 seconds, 20% O2 alternating 60 seconds, 8 hrs per day, 8 weeks, n=30); G5: Swimming training+ Intermittent hypoxia+ Salidroside (Conditioning 8 weeks+ Hypoxia: 8 weeks and treated 10mg/kg salidroside by oral gavage in 2 nd 4 weeks, 8 weeks, n=30)]。Blood pressure, cortex Fas and mitochondria dependent apoptotic and Endo G/ AIF signaling pathways will be measured. |
