Ethyl acetate extract of Wedelia chinensis inhibits tert-butyl hydroperoxide-induced damage in PC12 cells and D-galactose-induced neuronal cell loss in mice

doi:10.1186/1472-6882-14-491

中山醫學大學機構典藏 CSMUIR > 醫學院 > 醫學系 > 期刊論文 > Item 310902500/10194

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题名:	Ethyl acetate extract of Wedelia chinensis inhibits tert-butyl hydroperoxide-induced damage in PC12 cells and D-galactose-induced neuronal cell loss in mice
作者:	Wea-Lung Lin Wang, Shao-Ming Ho, Ying-Jui Kuo, Hsing-Chun Lee, Yean-Jang Tseng, Tsui-Hwa
贡献者:	醫學研究所
关键词:	Apoptosis t-butylhydroperoxide D-galactose Luteolin Wedelia chinensis Wedelolactone
日期:	2014-12-10
上传时间:	2015-02-13T04:45:06Z (UTC)
出版者:	BioMed Central
ISSN:	1472-6882
摘要:	Background Wedelia chinensis is traditionally used as a hepatoprotective herb in Taiwan. The aim of this study was to evaluate the neuroprotective potential of W. chinensis. Methods An ethyl acetate extract of W. chinensis (EAW) was prepared and analyzed by HPLC. The neuroprotective potential of EAW was assessed by tert-butylhydroperoxide (t-BHP)-induced damage in PC12 cells and D-galactose-induced damage in mouse cortex. Results EAW exhibited potent radical scavenging property and highly contained luteolin and wedelolactone. EAW decreased t-BHP-induced reactive oxygen species (ROS) accumulation, cytotoxicity and apoptosis in PC12 cells. EAW and its major constituents blocked t-BHP-induced cytochrome C release and Bcl-2 family protein ratio change. EAW and its major constituents increased the endogenous antioxidant capacity evaluated by the binding activity assay of nuclear factor E2-related factor 2 (Nrf2) to antioxidant response element (ARE) and nuclear translocation of Nrf2 respectively in PC12 cells. Finally, EAW inhibited D-galactose-induced lipid peroxidation, apoptosis and neuron loss in the cerebral cortex of mice. Conclusion These results demonstrate that W. chinensis has neuroprotective potential through blocking oxidative stress-induced damage and that luteolin and wedelolactone contribute to the protective action.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/10194 http://dx.doi.org/10.1186/1472-6882-14-491
關聯:	BMC Complementary and Alternative Medicine 2014, 14:491
显示于类别:	[醫學系] 期刊論文

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